CD48 Antibody (YA4688)

Cat. No.: HY-P84996: Data Sheet User Guide for Antibodies
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CD48 Antibody (YA4688) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to CD48.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

CD48 Antibody (YA4688) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to CD48.

Host

Mouse

Clonality

Monoclonal

Species Reactivity

Human

SwissProt ID

P09326

Gene ID

962 [NCBI]

Immunogen

Purified recombinant Human CD48

Application &
Dilution Ratio

Application	Dilution Ratio
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:5000-100000
FC FC: Flow Cytometry	1-2μg/Test

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in Phosphate-buffered solution.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein that interacts via its N-terminal immunoglobulin domain with cell surface receptors including CD244/2B4 or CD2 to regulate immune cell function and activation (PubMed:12007789, PubMed:19494291, PubMed:27249817, PubMed:9841922). Participates in T-cell signaling transduction by associating with CD2 and efficiently bringing the Src family protein kinase LCK and LAT to the TCR/CD3 complex (PubMed:19494291). In turn, promotes LCK phosphorylation and subsequent activation (PubMed:12007789). Induces the phosphorylation of the cytoplasmic immunoreceptortyrosine switch motifs (ITSMs) of CD244 initiating a series of signaling events that leads to the generation of the immunological synapse and the directed release of cytolytic granules containing perforin and granzymes by T-lymphocytes and NK-cells (PubMed:27249817)

Subcellular Localization：Cell membrane; Lipid-anchor, GPI-anchor; Membrane raft; Secreted

Expression：
Tissue_specificity:Widely expressed in all hematopoietic cells

Induction:By IFN-alpha/beta and IFN-gamma both at the level of CD48 mRNA and cell surface expression

Isoforms & Post-Translational Modification：P09326 has 2 isomers: P09326-1: 27683 Da (predicted); P09326-2: 19429 Da (predicted).

Subunit：Interacts with CD2 (PubMed:19494291). Interacts with CD244; this interaction is possible not only on different cells (trans interaction) but also on the same cell (cis interaction) (PubMed:27249817, PubMed:9841922). Interacts with LCK (PubMed:12007789)

Synonyms

CD48; BCM1; BLAST1; CD48 antigen; B-lymphocyte activation marker BLAST-1; BCM1 surface antigen; Leukocyte antigen MEM-102; TCT.1; CD48; CD48 nanobody;

Documentation

User Guide for Antibodies (1077 KB)