CD86 Antibody (YA10049)
(Synonyms: Interferon gamma; IFN-gamma; Immune interferon)
Based on 1 Customer Validation
CD86 Antibody (YA10049) is a Rat-derived and non-conjugated IgG2a Monoclonal antibody, targeting to CD86.
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Host:
Rat
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Isotype:
IgG2a
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Application:
ICC/IF, FC
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Reactivity :
Mouse
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Formulation:
Supplied in PBS (pH 7.3), containing 0.02% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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|---|---|---|
| Dilution Ratio | 1:50-200 | 1:500-2000 |
Product Details
CD86 Antibody (YA10049) is a Rat-derived and non-conjugated IgG2a Monoclonal antibody, targeting to CD86.
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Host Rat
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Clonality Monoclonal
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Species ReactivityMouse
LPS-activated CBA/Ca mouse splenic B cells
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG2a
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.3), containing 0.02% sodium azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CD86 is a Costimulatory molecule that belongs to the immunoglobulin superfamily that plays an important role in T-lymphocyte activation (By similarity). Acts as the primary auxiliary signal augmenting the MHC/TCR signal in naive T-cells by acting as a ligand for the CD28 receptor which is constitutively expressed on the cell surface of T-cells (By similarity). May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (By similarity). Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation. Also acts as an inhibitor of T-cell activation by acting as a ligand for CTLA4, a decoy receptor, thereby blocking CD28-mediated T-cell priming[1][2][3].
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Subcellular Localization
Cell membrane
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Expression
Tissue_Specificity: Expressed on the surfaces of antigen-presenting cells. -
Isoforms & Post-Translational Modification
CD86 has 2 isoforms, P42082-1: amino acid length is 309, molecular weight is 34666 Da (predicted); P42082-2: amino acid length is 303, molecular weight is 33962 Da (predicted).Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation
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Subunit
Homodimer.
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SwissProt ID
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Synonyms
Interferon gamma; IFN-gamma; Immune interferon
Documentation
[1]. Lucas CR, et al. Prohibitins and the cytoplasmic domain of CD86 cooperate to mediate CD86 signaling in B lymphocytes. J Immunol. 2013 Jan 15;190(2):723-36. [Content Brief]
[2]. Freeman GJ, et al. Murine B7-2, an alternative CTLA4 counter-receptor that costimulates T cell proliferation and interleukin 2 production. J Exp Med. 1993 Dec 1;178(6):2185-92. [Content Brief]
[3]. Chen C, et al. Molecular cloning and expression of early T cell costimulatory molecule-1 and its characterization as B7-2 molecule. J Immunol. 1994 May 15;152(10):4929-36. [Content Brief]
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