CFI Antibody (YA8110)(PBS only)

Cat. No.: HY-P88426A: Data Sheet User Guide for Antibodies
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CFI Antibody (YA8110) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to CFI.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

CFI Antibody (YA8110) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to CFI.

Host

Mouse

Molecular Weight

Predicted band size: 63.4 kDa

Species Reactivity

Human

SwissProt ID

P05156

Immunogen

Human recombinant protein fragment corresponding to amino acids 340-583 of human CFI produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:2000-2500

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG2b

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Trypsin-like serine protease that plays an essential role in regulating the immune response by controlling all complement pathways. Inhibits these pathways by cleaving three peptide bonds in the alpha-chain of C3b and two bonds in the alpha-chain of C4b thereby inactivating these proteins (PubMed:17320177, PubMed:7360115). Essential cofactors for these reactions include factor H and C4BP in the fluid phase and membrane cofactor protein/CD46 and CR1 on cell surfaces (PubMed:12055245, PubMed:2141838, PubMed:9605165). The presence of these cofactors on healthy cells allows degradation of deposited C3b by CFI in order to prevent undesired complement activation, while in apoptotic cells or microbes, the absence of such cofactors leads to C3b-mediated complement activation and subsequent opsonization (PubMed:28671664)

Subcellular Localization：Secreted, extracellular space,Secreted

Expression：
Tissue_Specificity: Expressed in the liver by hepatocytes (PubMed:6327681). Also present in other cells such as monocytes, fibroblasts or keratinocytes (PubMed:17320177, PubMed:6444659)

Isoforms & Post-Translational Modification：P05156: 583 amino acids, molecular weight 65750 Da.

Subunit：Heterodimer of a light and heavy chains; disulfide-linked

Synonyms

AHUS3; ARMD13; C3b-INA; C3BINA; FI; IF; KAF

Documentation

User Guide for Antibodies (1077 KB)