MAP3K8 Antibody (YA8246)

Cat. No.: HY-P88562: Data Sheet User Guide for Antibodies
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MAP3K8 Antibody (YA8246) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MAP3K8.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

MAP3K8 Antibody (YA8246) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MAP3K8.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 52.7 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P41279

Immunogen

Human recombinant protein fragment corresponding to amino acids 277-461 of human MAP3K8 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Required for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the pro-inflammatory cytokine TNF-alpha (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4. Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle. Isoform 1 shows some transforming activity, although it is much weaker than that of the activated oncogenic variant

Subcellular Localization：Cytoplasm

Expression：
Tissue_Specificity: Expressed in several normal tissues and human tumor-derived cell lines
Induction: Up-regulated by IL12 in T-lymphocytes. Up-regulated in subcutaneous adipose tissue of obese individuals

Isoforms & Post-Translational Modification：P41279 has two isomers: P41279-1: 52925 Da (predicted); P41279-2: 49620 Da (predicted).
Autophosphorylated (PubMed:1833717, PubMed:8226782)

Subunit：Forms a ternary complex with NFKB1/p105 and TNIP2

Synonyms

AURA2; c-COT; COT; EST; ESTF; MEKK8; Tpl-2; TPL2

Documentation

User Guide for Antibodies (1077 KB)