phospho-Girdin (Tyr1765) Antibody (YA7228)

Cat. No.: HY-P87544: Data Sheet User Guide for Antibodies
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phospho-Girdin (Tyr1765) Antibody (YA7228) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to phospho-Girdin (Tyr1765).

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

phospho-Girdin (Tyr1765) Antibody (YA7228) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to phospho-Girdin (Tyr1765).

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 206 kDa;

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q3V6T2

Immunogen

KLH conjugated synthesised phosphopeptide derived from human APE around the phosphorylation site of Ser1765

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1000
IHC-F IHC-F: Immunohistochemistry-Frozen	1:200-1000
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:200-1000

Sensitivity	Endogenous	Purity	affinity purified by Protein A
Conjugation	Non-conjugated	Modification	Phosphorylated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 0.01M tris buffered saline (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Bifunctional modulator of guanine nucleotide-binding proteins (G proteins) (PubMed:19211784, PubMed:27621449). Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates guanine nucleotide-binding protein G(i) alpha subunits (PubMed:19211784, PubMed:21954290, PubMed:23509302, PubMed:25187647). Also acts as a guanine nucleotide dissociation inhibitor for guanine nucleotide-binding protein G(s) subunit alpha GNAS (PubMed:27621449). Essential for cell migration (PubMed:16139227, PubMed:19211784, PubMed:20462955, PubMed:21954290). Interacts in complex with G(i) alpha subunits with the EGFR receptor, retaining EGFR at the cell membrane following ligand stimulation and promoting EGFR signaling which triggers cell migration (PubMed:20462955). Binding to Gi-alpha subunits displaces the beta and gamma subunits from the heterotrimeric G-protein complex which enhances phosphoinositide 3-kinase (PI3K)-dependent phosphorylation and kinase activity of AKT1/PKB (PubMed:19211784). Phosphorylation of AKT1/PKB induces the phosphorylation of downstream effectors GSK3 and FOXO1/FKHR, and regulates DNA replication and cell proliferation (By similarity). Binds in its tyrosine-phosphorylated form to the phosphatidylinositol 3-kinase (PI3K) regulatory subunit PIK3R1 which enables recruitment of PIK3R1 to the EGFR receptor, enhancing PI3K activity and cell migration (PubMed:21954290). Plays a role as a key modulator of the AKT-mTOR signaling pathway, controlling the tempo of the process of newborn neuron integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation (By similarity). Inhibition of G(s) subunit alpha GNAS leads to reduced cellular levels of cAMP and suppression of cell proliferation (PubMed:27621449). Essential for the integrity of the actin cytoskeleton (PubMed:16139227, PubMed:19211784). Required for formation of actin stress fibers and lamellipodia (PubMed:15882442). May be involved in membrane sorting in the early endosome (PubMed:15882442). Plays a role in ciliogenesis and cilium morphology and positioning and this may partly be through regulation of the localization of scaffolding protein CROCC/Rootletin (PubMed:27623382)

Subcellular Localization：Cell membrane,Cytoplasm, cytosol,Cytoplasmic vesicle,Cell projection, lamellipodium,Cytoplasm, cytoskeleton, cilium basal body,Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole

Expression：
Tissue_Specificity: Expressed ubiquitously

Isoforms & Post-Translational Modification：Q3V6T2 has 5 isomers: Q3V6T2-1: 216042 Da (predicted); Q3V6T2-2: 212620 Da (predicted); Q3V6T2-3: 215954 Da (predicted); Q3V6T2-4: 212533 Da (predicted); Q3V6T2-5: 207797 Da (predicted).
Phosphorylation is induced by epidermal growth factor (EGF) in a phosphoinositide 3-kinase (PI3K)-dependent manner (PubMed:16139227)

Subunit：Homodimer (PubMed:16139227)

Documentation

User Guide for Antibodies (1077 KB)