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Phospho-IKB alpha (Ser32) Antibody (YA183) (PBS only)

(Synonyms: IKBA, MAD3, NFKBI, NFKBIA, NF-kappa-B inhibitor alpha, I-kappa-B-alpha, Major histocompatibility complex enhancer-binding protein MAD3, IkB-alpha, IkappaBalpha)

Phospho-IKB alpha (Ser32) Antibody (YA183) (PBS only) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-IKB alpha (Ser32).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in PBS (pH7.4).

  • Conjugation:
    Non-conjugated
  • Phospho-IKB alpha (Ser32) Antibody (YA183) (PBS only) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:1000 1:50-1:200 Use at an assay dependent concentration.

Product Details

Description

Phospho-IKB alpha (Ser32) Antibody (YA183) (PBS only) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-IKB alpha (Ser32).

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 36 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 36 kDa
Species Reactivity Database
Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser32 of Human IKB alpha.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Phosphorylated

Isotype

IgG

RRID

AB_3102540

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4).

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Background

  • Function

    IKB alpha inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL (RELA/p65 and NFKB1/p50) dimers in the cytoplasm by masking their nuclear localization signals. On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription[1][2][3][4][5][6].

  • Subcellular Localization

    Cytoplasm,Nucleus

  • Expression

    Induction: Induced in adherent monocytes

  • Isoforms & Post-Translational Modification

    P25963: 317 amino acids, molecular weight 35609 Da.

  • Subunit

    Interacts with RELA; the interaction requires the nuclear import signal

  • SwissProt ID

    P25963

  • Gene ID
    4792 [NCBI]
  • Synonyms

    IKBA, MAD3, NFKBI, NFKBIA, NF-kappa-B inhibitor alpha, I-kappa-B-alpha, Major histocompatibility complex enhancer-binding protein MAD3, IkB-alpha, IkappaBalpha

  • Research Field

    Signal Transduction

Phospho-IKB alpha (Ser32) Antibody (YA183) (PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • Phospho-IKB alpha (Ser32) Antibody (YA183) (PBS only) Product Image
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  • Phospho-IKB alpha (Ser32) Antibody (YA183) (PBS only) Product Image