PIKFYVE Antibody (YA8175)(PBS only)
(Synonyms: CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29)PIKFYVE Antibody (YA8175) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PIKFYVE.
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Host:
Mouse
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Isotype:
IgG
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Application:
IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|
| Dilution Ratio | 1:150-500 |
Product Details
PIKFYVE Antibody (YA8175) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PIKFYVE.
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Host Mouse
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Species ReactivityHuman, Mouse, Rat
Full length human recombinant protein of human PIKFYVE produced in E.coli.
Affinity purified
Non-conjugated
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
PIKfyve, a FYVE finger-containing phosphoinositide kinase, is an enzyme that in humans is encoded by the PIKFYVE gene. The principal enzymatic activity of PIKfyve is to phosphorylate PtdIns3P to PtdIns (3,5) P2. PIKfyve activity is responsible for the production of both PtdIns (3,5) P2 and phosphatidylinositol 5-phosphate (PtdIns5P) . PIKfyve is a large protein, containing a number of functional domains and expressed in several spliced forms. By directly binding membrane PtdIns (3) P, the FYVE finger domain of PIKfyve is essential in localizing the protein to the cytosolic leaflet of endosomes. Impaired PIKfyve enzymatic activity by dominant-interfering mutants, siRNA- mediated ablation or pharmacological inhibition causes endosome enlargement and cytoplasmic vacuolation due to impaired PtdIns (3,5) P2 synthesis. Thus, via PtdIns (3,5) P2 production, PIKfyve participates in several aspects of endosome dynamics, thereby affecting a number of trafficking pathways that emanate from or traverse the endosomal system en route to the trans-Golgi network or later compartments along the endocytic pathway.
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Subcellular Localization
Endosome membrane,Early endosome membrane,Cytoplasmic vesicle, phagosome membrane,Late endosome membrane
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Isoforms & Post-Translational Modification
Q9Y2I7 has four isomers: Q9Y2I7-1: 237136 Da (predicted); Q9Y2I7-2: 50211 Da (predicted); Q9Y2I7-3: 51476 Da (predicted); Q9Y2I7-4: 61595 Da (predicted).
Autophosphorylates which inhibits its own phosphatidylinositol 3-phosphate 5-kinase activity, stimulates FIG4 lipid phosphatase activity and down-regulates lipid product formation (PubMed:33098764) -
Subunit
Component of the PI(3,5)P2 regulatory complex/PAS complex, at least composed of PIKFYVE, FIG4 and VAC14
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SwissProt ID
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Synonyms
CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29
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