PPAP2A Antibody (YA8025)

Cat. No.: HY-P88341: Data Sheet User Guide for Antibodies
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PPAP2A Antibody (YA8025) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PPAP2A.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

PPAP2A Antibody (YA8025) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PPAP2A.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 32 kDa

Species Reactivity

Human

SwissProt ID

O14494

Immunogen

Full length human recombinant protein of human PPAP2A produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150-500

Sensitivity	Endogenous	Purity	Affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Magnesium-independent phospholipid phosphatase of the plasma membrane that catalyzes the dephosphorylation of a variety of glycerolipid and sphingolipid phosphate esters including phosphatidate/PA, lysophosphatidate/LPA, diacylglycerol pyrophosphate/DGPP, sphingosine 1-phosphate/S1P and ceramide 1-phosphate/C1P (PubMed:10962286, PubMed:17379599, PubMed:9305923, PubMed:9607309, PubMed:9705349). Also acts on N-oleoyl ethanolamine phosphate/N-(9Z-octadecenoyl)-ethanolamine phosphate, a potential physiological compound (PubMed:9607309). Through its extracellular phosphatase activity allows both the hydrolysis and the cellular uptake of these bioactive lipid mediators from the milieu, regulating signal transduction in different cellular processes (PubMed:10962286, PubMed:12909631, PubMed:15461590, PubMed:17379599). It is for instance essential for the extracellular hydrolysis of S1P and subsequent conversion into intracellular S1P (PubMed:17379599). Involved in the regulation of inflammation, platelets activation, cell proliferation and migration among other processes (PubMed:12909631, PubMed:15461590). May also have an intracellular activity to regulate phospholipid-mediated signaling pathways (By similarity)

Subcellular Localization：Cell membrane,Apical cell membrane,Membrane raft,Membrane, caveola

Expression：
Tissue_Specificity: Widely expressed with highest expression found in prostate (PubMed:9305923). Found to be down-regulated in colon adenocarcinomas (PubMed:9570154)|Predominant in kidney, lung, placenta and liver|Predominant in heart and pancreas
Induction: By androgens

Isoforms & Post-Translational Modification：O14494 has two isomers: O14494-1: 32156 Da (predicted); O14494-2: 32150 Da (predicted).
N-glycosylated (PubMed:10962286, PubMed:9305923)

Subunit：Forms functional homodimers and homooligomers that are not required for substrate recognition and catalytic activity (By similarity)

Synonyms

LLP1a; LPP1; PAP-2a; PAP2; PPAP2A

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/bmdc-cell.html [Content Brief]