RBFOX2 Antibody (YA3534)

Cat. No.: HY-P83837: Data Sheet User Guide for Antibodies
FAQs
Technical Support

RBFOX2 Antibody (YA3534) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RBFOX2.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
50 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
100 μL	Get quote 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
250 μL	Get quote

Synthetic products have potential research and development risk.

or Bulk Inquiry

* Please select Quantity before adding items.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation
References

Description

RBFOX2 Antibody (YA3534) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RBFOX2.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 41.4 kDa;

Observed band size: 43 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

O43251

Gene ID

23543 [NCBI]

Immunogen

Purified recombinant fragment of human RBFOX2 (AA: 1-145) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Solution

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Flow cytometric analysis of 1X10^6 HL-60 cells labeling RBFOX2 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

Function：RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Prevents binding of U2AF2 to the 3'-splice site. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis (By similarity). RNA-binding protein that seems to act as a coregulatory factor of ER-alpha. Together with RNA binding proteins RBPMS and MBNL1/2, activates vascular smooth muscle cells alternative splicing events (PubMed:37548402)

Subcellular Localization：Nucleus; Cytoplasm

Isoforms & Post-Translational Modification：O43251 has 10 isomers: O43251-1: 41374 Da (predicted); O43251-2: 40479 Da (predicted); O43251-3: 37879 Da (predicted); O43251-4: 39783 Da (predicted); O43251-5: 39954 Da (predicted); O43251-6: 47215 Da (predicted); O43251-7: 43179 Da (predicted); O43251-8: 47343 Da (predicted); O43251-9: 39515 Da (predicted); O43251-10: 40351 Da (predicted).

Subunit：Interacts with ER-alpha N-terminal activation domain. Interacts with RBPMS; the interaction allows cooperative assembly of stable cell-specific alternative splicing regulatory complexes (PubMed:37548402)

Synonyms

RTA; fxh; FOX2; RBM9; Fox-2; HNRBP2; HRNBP2; dJ106I20.3

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/nci-h460-cell.html [Content Brief]