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RBFOX2 Antibody (YA3534)

(Synonyms: RTA; fxh; FOX2; RBM9; Fox-2; HNRBP2; HRNBP2; dJ106I20.3)

RBFOX2 Antibody (YA3534) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RBFOX2.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    IHC-P, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for RBFOX2 Antibody (YA3534)
  • RBFOX2 Antibody (YA3534) Product Image
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Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

RBFOX2 Antibody (YA3534) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RBFOX2.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 43 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 41.4 kDa
Immunogen

Purified recombinant fragment of human RBFOX2 (AA: 1-145) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for RBFOX2 Antibody (YA3534)
    Flow cytometric analysis of 1X10^6 HL-60 cells labeling RBFOX2 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    RBFOX2 is a RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Prevents binding of U2AF2 to the 3'-splice site. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis. RNA-binding protein that seems to act as a coregulatory factor of ER-alpha. Together with RNA binding proteins RBPMS and MBNL1/2, activates vascular smooth muscle cells alternative splicing events[1].

  • Subcellular Localization

    Nucleus; Cytoplasm

  • Isoforms & Post-Translational Modification

    O43251 has 10 isomers: O43251-1: 41374 Da (predicted); O43251-2: 40479 Da (predicted); O43251-3: 37879 Da (predicted); O43251-4: 39783 Da (predicted); O43251-5: 39954 Da (predicted); O43251-6: 47215 Da (predicted); O43251-7: 43179 Da (predicted); O43251-8: 47343 Da (predicted); O43251-9: 39515 Da (predicted); O43251-10: 40351 Da (predicted).

  • Subunit

    Interacts with ER-alpha N-terminal activation domain. Interacts with RBPMS; the interaction allows cooperative assembly of stable cell-specific alternative splicing regulatory complexes (PubMed:37548402)

  • SwissProt ID

    O43251

  • Gene ID
    23543 [NCBI]
  • Synonyms

    RTA; fxh; FOX2; RBM9; Fox-2; HNRBP2; HRNBP2; dJ106I20.3

RBFOX2 Antibody (YA3534) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 HL-60 cells labeling RBFOX2 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • RBFOX2 Antibody (YA3534) Product Image
    1/16
  • Experimental Validation Results for RBFOX2 Antibody (YA3534)
  • RBFOX2 Antibody (YA3534) Product Image
  • Experimental Validation Results for RBFOX2 Antibody (YA3534)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 HL-60 cells labeling RBFOX2 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for RBFOX2 Antibody (YA3534)
  • RBFOX2 Antibody (YA3534) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 HL-60 cells labeling RBFOX2 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • RBFOX2 Antibody (YA3534) Product Image FC