RUNX3 Antibody (YA1141)(PBS only)

Cat. No.: HY-P81396A: Data Sheet User Guide for Antibodies
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RUNX3 Antibody (YA1141) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RUNX3.

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사이즈		재고
50 μL		견적 받기
100 μL		견적 받기

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
제품 설명

제품 설명

RUNX3 Antibody (YA1141) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RUNX3.

Host

Rabbit

분자량

Predicted band size: 44 kDa;

Observed band size: 44 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q13761

Gene ID

864 [NCBI]

Immunogen

Recombinant protein of human RUNX3

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-1:200

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Background

Function：Forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters (By similarity). May be involved in the control of cellular proliferation and/or differentiation. In association with ZFHX3, up-regulates CDKN1A promoter activity following TGF-beta stimulation (PubMed:20599712). CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing (By similarity). Necessary for the development and survival of sensory neurons expressing parvalbumin (By similarity)

Subcellular Localization：Nucleus; Cytoplasm

Expression：
Tissue_specificity:Expression (protein level) in gastric cancer tissue

Isoforms & Post-Translational Modification：Q13761 has 2 isomers: Q13761-1: 44356 Da (predicted); Q13761-2: 45922 Da (predicted).
Phosphorylated on tyrosine residues by SRC. Phosphorylated by LCK and FYN

Subunit：Heterodimer with CBFB. Interacts with TLE1 and SUV39H1 (PubMed:16652147, PubMed:9751710).

Research Field

Epigenetics and Nuclear Signaling

Synonyms

AML2; CBFA3; PEBP2aC; FLJ34510; MGC16070

각종 서류

User Guide for Antibodies (1077 KB)