PAI1 Antibody (YA3653)

Cat. No.: HY-P83956: Data Sheet COA
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User Guide for Antibodies
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PAI1 Antibody (YA3653) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PAI1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

PAI1 Antibody (YA3653) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to PAI1.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 45 kDa;

Observed band size: 45 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat, Rabbit

SwissProt ID

P05121

Gene ID

5054 [NCBI]

Immunogen

Purified recombinant fragment of human SERPINE1 aa 194-316.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Serine protease inhibitor. Inhibits TMPRSS7 (PubMed:15853774). Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots (PubMed:17912461, PubMed:8481516, PubMed:9207454, PubMed:21925150). As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading (PubMed:9175705). Acts as a regulator of cell migration, independently of its role as protease inhibitor (PubMed:15001579, PubMed:9168821). It is required for stimulation of keratinocyte migration during cutaneous injury repair (PubMed:18386027). It is involved in cellular and replicative senescence (PubMed:16862142). Plays a role in alveolar type 2 cells senescence in the lung (By similarity). Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis (PubMed:25808697, PubMed:27046084)

Subcellular Localization：Secreted

Expression：
Tissue_specificity:It is expressed in endothelial cells (PubMed:2430793, PubMed:3097076) . It is present in plasma, platelets, and hepatocellular carcinoma and fibrosarcoma cells.

Induction:Up-regulated by coagulation factor Xa (F10) in atrial tissues (PubMed:24041930) . Up-regulated in atrial tissues by rapid pacing resembling atrial fibrillation (PubMed:24041930)

Isoforms & Post-Translational Modification：P05121 has 2 isomers: P05121-1: 45060 Da (predicted); P05121-2: 43404 Da (predicted).
Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-|-Met-370 bond

Subunit：Forms a heterodimer with TMPRSS7 (PubMed:15853774). Interacts with VTN (PubMed:7522053). Binds LRP1B; binding is followed by internalization and degradation (PubMed:11384978).

RRID

AB_3718802

Synonyms

PAI; PAI1; PAI-1; PLANH1

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/pc-3-cell.html [Content Brief]