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TSH Receptor/TSH-R Antibody (YA1252)

(Synonyms: LGR3; TSHR; Thyrotropin Receptor; Thyroid-stimulating hormone receptor (TSH-R))
Customer Review

Based on 1 Customer Validation

TSH Receptor/TSH-R Antibody (YA1252) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TSH Receptor/TSH-R.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    IHC-P

  • Reactivity :

    Human

  • Formulation:

    Supplied as a 0.22 μm filtered solution of 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
    Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

  • Conjugation:
    Non-conjugated
IHC-P
  • Experimental Validation Results for TSH Receptor/TSH-R Antibody (YA1252)
  • Experimental Validation Results for TSH Receptor/TSH-R Antibody (YA1252)
  • Experimental Validation Results for TSH Receptor/TSH-R Antibody (YA1252)
  • Experimental Validation Results for TSH Receptor/TSH-R Antibody (YA1252)
  • Experimental Validation Results for TSH Receptor/TSH-R Antibody (YA1252)
  • Experimental Validation Results for TSH Receptor/TSH-R Antibody (YA1252)
  • TSH Receptor/TSH-R Antibody (YA1252) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000

Product Details

Description

TSH Receptor/TSH-R Antibody (YA1252) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TSH Receptor/TSH-R.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 86 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 86 kDa
Species Reactivity Database

Entrez Gene: 7253 Human

SwissProt: P16473 Human

Immunogen

Recombinant protein of human TSH Receptor

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied as a 0.22 μm filtered solution of 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
    Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TSH Receptor/TSH-R Antibody (YA1252)
    Immunohistochemical analysis of paraffin-embedded human Thyroid Cancer tissue using TSH Receptor/TSH-R antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81507, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TSH Receptor/TSH-R Antibody (YA1252)
    Immunohistochemical analysis of paraffin-embedded human Thyroid Cancer tissue using TSH Receptor/TSH-R antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81507, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TSH Receptor/TSH-R Antibody (YA1252)
    Immunohistochemical analysis of paraffin-embedded human Thyroid Cancer tissue using TSH Receptor/TSH-R antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81507, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TSH Receptor/TSH-R Antibody (YA1252)
    Immunohistochemical analysis of paraffin-embedded human Thyroid Cancer tissue using TSH Receptor/TSH-R antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81507, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TSH Receptor/TSH-R Antibody (YA1252)
    Immunohistochemical analysis of paraffin-embedded human Thyroid Cancer tissue using TSH Receptor/TSH-R antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81507, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TSH Receptor/TSH-R Antibody (YA1252)
    Immunohistochemical analysis of paraffin-embedded human Thyroid Cancer tissue using TSH Receptor/TSH-R antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81507, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

Background

  • Function

    TSH Receptor/TSH-R receptor for the thyroid-stimulating hormone (TSH) or thyrotropin. Also acts as a receptor for the heterodimeric glycoprotein hormone (GPHA2:GPHB5) or thyrostimulin. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Plays a central role in controlling thyroid cell metabolism[1][2].

  • Subcellular Localization

    Cell membrane; Multi-pass membrane protein; Basolateral cell membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Expressed in thyroide cells (at protein level) (PubMed:11847099) . Expressed in the thyroid (PubMed:2610690)

  • Isoforms & Post-Translational Modification

    P16473 has 3 isomers: P16473-1: 86844 Da (predicted); P16473-2: 28427 Da (predicted); P16473-3: 30800 Da (predicted).
    Glycosylated;Sulfated. Sulfation on Tyr-385 plays a role in thyrotropin receptor binding and activation

  • Subunit

    Interacts with heterodimer GPHA2:GPHB5; this interaction stimulates cAMP production (PubMed:12045258). Interacts (via the PDZ-binding motif) with SCRIB; regulates TSHR trafficking and function (PubMed:15775968)

  • SwissProt ID

    P16473

  • Gene ID
    7253 [NCBI]
  • Synonyms

    LGR3; TSHR; Thyrotropin Receptor; Thyroid-stimulating hormone receptor (TSH-R)

  • Research Field

    Signal Transduction

TSH Receptor/TSH-R Antibody (YA1252) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Thyroid Cancer tissue using TSH Receptor/TSH-R antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81507, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Thyroid Cancer tissue using TSH Receptor/TSH-R antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81507, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Thyroid Cancer tissue using TSH Receptor/TSH-R antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81507, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Thyroid Cancer tissue using TSH Receptor/TSH-R antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81507, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Thyroid Cancer tissue using TSH Receptor/TSH-R antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81507, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Thyroid Cancer tissue using TSH Receptor/TSH-R antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81507, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • TSH Receptor/TSH-R Antibody (YA1252) Product Image
    1/16
  • Experimental Validation Results for TSH Receptor/TSH-R Antibody (YA1252)
  • Experimental Validation Results for TSH Receptor/TSH-R Antibody (YA1252)
  • Experimental Validation Results for TSH Receptor/TSH-R Antibody (YA1252)
  • Experimental Validation Results for TSH Receptor/TSH-R Antibody (YA1252)
  • Experimental Validation Results for TSH Receptor/TSH-R Antibody (YA1252)
  • Experimental Validation Results for TSH Receptor/TSH-R Antibody (YA1252)
  • TSH Receptor/TSH-R Antibody (YA1252) Product Image
  • Experimental Validation Results for TSH Receptor/TSH-R Antibody (YA1252)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Thyroid Cancer tissue using TSH Receptor/TSH-R antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81507, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TSH Receptor/TSH-R Antibody (YA1252)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Thyroid Cancer tissue using TSH Receptor/TSH-R antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81507, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TSH Receptor/TSH-R Antibody (YA1252)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Thyroid Cancer tissue using TSH Receptor/TSH-R antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81507, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TSH Receptor/TSH-R Antibody (YA1252)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Thyroid Cancer tissue using TSH Receptor/TSH-R antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81507, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TSH Receptor/TSH-R Antibody (YA1252)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Thyroid Cancer tissue using TSH Receptor/TSH-R antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81507, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TSH Receptor/TSH-R Antibody (YA1252)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human Thyroid Cancer tissue using TSH Receptor/TSH-R antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81507, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.
  • Experimental Validation Results for TSH Receptor/TSH-R Antibody (YA1252)
  • Experimental Validation Results for TSH Receptor/TSH-R Antibody (YA1252)
  • Experimental Validation Results for TSH Receptor/TSH-R Antibody (YA1252)
  • Experimental Validation Results for TSH Receptor/TSH-R Antibody (YA1252)
  • Experimental Validation Results for TSH Receptor/TSH-R Antibody (YA1252)
  • Experimental Validation Results for TSH Receptor/TSH-R Antibody (YA1252)
  • TSH Receptor/TSH-R Antibody (YA1252) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Thyroid Cancer tissue using TSH Receptor/TSH-R antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81507, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • TSH Receptor/TSH-R Antibody (YA1252) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Thyroid Cancer tissue using TSH Receptor/TSH-R antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81507, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • TSH Receptor/TSH-R Antibody (YA1252) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Thyroid Cancer tissue using TSH Receptor/TSH-R antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81507, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • TSH Receptor/TSH-R Antibody (YA1252) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Thyroid Cancer tissue using TSH Receptor/TSH-R antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81507, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • TSH Receptor/TSH-R Antibody (YA1252) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Thyroid Cancer tissue using TSH Receptor/TSH-R antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81507, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • TSH Receptor/TSH-R Antibody (YA1252) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human Thyroid Cancer tissue using TSH Receptor/TSH-R antibody was performed. The section was pretreated using high-temperature and high-pressure mediated EDTA antigen retrieval buffer (pH 9.0), for 5 minutes. The tissues were incubated with primary antibody (HY-P81507, 1:500 dilution) at room temperature for 60 minutes. Detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. The tissues were counterstained with hematoxylin and mounted with neutral balsam mounting medium.

  • TSH Receptor/TSH-R Antibody (YA1252) Product Image IHC-P
  • TSH Receptor/TSH-R Antibody (YA1252) Product Image IHC-P
  • TSH Receptor/TSH-R Antibody (YA1252) Product Image IHC-P
  • TSH Receptor/TSH-R Antibody (YA1252) Product Image IHC-P
  • TSH Receptor/TSH-R Antibody (YA1252) Product Image IHC-P
  • TSH Receptor/TSH-R Antibody (YA1252) Product Image IHC-P