Anticancer agent-363
Anticancer agent-363 is an anticancer agent that exhibits cytotoxic activity against cancer cells and is selective for non-cancerous fibroblasts. Anticancer agent-363 has the highest binding affinity for c-Met protein. Anticancer agent-363 can be used for research on lung cancer and mammary adenocarcinoma.
For research use only. We do not sell to patients.
- Formula: C20H16BrClN6O4S
- Molecular Weight:551.80
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
0.29 μM
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Cytotoxicity against human A549 non-small cell lung carcinoma cells assessed as reduction in cell viability by MTT assay.
Cytotoxicity against human A549 non-small cell lung carcinoma cells assessed as reduction in cell viability by MTT assay.
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42643104 |
| MCF7 | IC50 |
3.1 μM
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Cytotoxicity against human MCF-7 breast adenocarcinoma cells assessed as reduction in cell viability by MTT assay.
Cytotoxicity against human MCF-7 breast adenocarcinoma cells assessed as reduction in cell viability by MTT assay.
|
42643104 |
| NIH3T3 | IC50 |
66.36 μM
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Cytotoxicity against normal noncancerous mouse embryonic fibroblast NIH/3T3 cells assessed as reduction in cell viability by MTT assay.
Cytotoxicity against normal noncancerous mouse embryonic fibroblast NIH/3T3 cells assessed as reduction in cell viability by MTT assay.
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42643104 |
In Vitro
Anticancer agent-363 (Compound 10d) exhibits the most potent anticancer activity against A549 (IC50 = 0.29 μM) and MCF-7 (IC50 = 3.1 μM) cell lines, and shows significantly lower toxicity toward normal NIH/3T3 fibroblasts (IC50 = 66.36 μM)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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Molecular Weight 551.80
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Formula C20H16BrClN6O4S
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SMILES
COC1=CC=C(NC(CSC2=NN=C(C3=CC(Br)=NN3C4=C(Cl)C=CC=N4)O2)=O)C(OC)=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anticancer agent-363 Related Classifications
HY-189410 Related Classifications
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