Art. -Nr.: HY-112217A

PSEM 89S TFA

Name: PSEM 89S TFA
Brand: MedChemExpress
SKU: HY-112217A
Price: 120.00 USD
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PSEM 89S TFA is a selective and brain penetrant agonists for the resulting ion channels. PSEM 89S TFA is orthogonally selective for Q79G and L141F, respectively.

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Reinheit : 99.85%
CAS. Nr.: 1336913-03-1
Formel: C₁₈H₂₃F₃N₂O₅
Molecular Weight:404.38

Speicherung:

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

PSEM 89S TFA Chemical Structure

CAS. Nr.: 1336913-03-1

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Biologische Aktivität
- Beschreibung
- In Vitro
- In Vivo
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

PSEM 89S TFA is a selective and brain penetrant agonists for the resulting ion channels. PSEM 89S TFA is orthogonally selective for Q79G and L141F, respectively^[1].

In Vitro

PSEM 89S (10 or 30μM) activates layer 2/3 cortical neurons expressing (Pharmacologically Selective Actuator Module) PSAM^L141F,Y115F-5HT3 high conductance (HC)^[1].
PSEM 89S (10 μM) reversibly silenced transfected neurons by reducing cellular input resistance^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

PSEM 89S strongly reduces photostimulation-evoked feeding in mice expressing PSAM^L141F,Y115F- glycine receptor (GlyR) (30 mg/kg) but not in mice expressing only channelrhodopsin-2 (ChR2) (50 mg/kg)^[1].
PSEM 89S (30 mg/kg) shows good brain penetrance in mice after minimally invasive intraperitoneal administration^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male Agrp-cre mice (3-6 weeks)^[1]
Dosage:

30 mg/kg
Administration:

I.p. administration
Result:

Almost completely suppressed fos (a marker of neuron activation) in ChR2-expressing neurons.

Animal Model:

C57BL/6 mice^[1]
Dosage:

30 mg/kg (Pharmacokinetic Analysis)
Administration:

I.p. administration
Result:

Rised rapidly in serum and brain, and was mostly cleared from both compartments in 1 h.

Chemical Information

CAS. Nr. 1336913-03-1
Appearance Solid
Molecular Weight 404.38
Formel C₁₈H₂₃F₃N₂O₅
Color Off-white to light yellow
SMILES

O=C(N[C@@H]1CN2CCC1CC2)C3=CC(OC)=CC=C3OC.O=C(O)C(F)(F)F
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

-20°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Lösungsmittel & Löslichkeit

In Vitro:

H₂O : ≥ 100 mg/mL (247.29 mM)

DMSO : 100 mg/mL (247.29 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.4729 mL

12.3646 mL

24.7292 mL

5 mM

0.4946 mL

2.4729 mL

4.9458 mL

10 mM

0.2473 mL

1.2365 mL

2.4729 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (6.18 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (6.18 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (6.18 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Christopher JM, et, al. Chemical and genetic engineering of selective ion channel-ligand interactions. Science. 2011 Sep 2; 333(6047): 1292-6. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	2.4729 mL	12.3646 mL	24.7292 mL	61.8230 mL
	5 mM	0.4946 mL	2.4729 mL	4.9458 mL	12.3646 mL
	10 mM	0.2473 mL	1.2365 mL	2.4729 mL	6.1823 mL
	15 mM	0.1649 mL	0.8243 mL	1.6486 mL	4.1215 mL
	20 mM	0.1236 mL	0.6182 mL	1.2365 mL	3.0912 mL
	25 mM	0.0989 mL	0.4946 mL	0.9892 mL	2.4729 mL
	30 mM	0.0824 mL	0.4122 mL	0.8243 mL	2.0608 mL
	40 mM	0.0618 mL	0.3091 mL	0.6182 mL	1.5456 mL
	50 mM	0.0495 mL	0.2473 mL	0.4946 mL	1.2365 mL
	60 mM	0.0412 mL	0.2061 mL	0.4122 mL	1.0304 mL
	80 mM	0.0309 mL	0.1546 mL	0.3091 mL	0.7728 mL
	100 mM	0.0247 mL	0.1236 mL	0.2473 mL	0.6182 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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PSEM 89S TFA