Art. -Nr.: HY-138097

α-NETA

Name: α-NETA
Brand: MedChemExpress
SKU: HY-138097
Price: 75.00 USD
Availability: InStock
Rating: 5 (13 reviews)

13 Cited Publications

Kundenbewertung

Based on 13 publication(s) in Google Scholar

α-NETA is a potent and noncompetitive choline acetyltransferase (ChA) inhibitor with an IC₅₀ of 9 μM. α-NETA is a potent ALDH1A1 (IC₅₀=0.04 µM) and chemokine-like receptor-1 (CMKLR1) antagonist. α-NETA weakly inhibits cholinesterase (ChE; IC₅₀=84 µM) and acetylcholinesterase (AChE; IC₅₀=300 µM). α-NETA has anti-cancer activity.

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Reinheit : ≥98.0%
CAS. Nr.: 115066-04-1
Formel: C₁₆H₂₀INO
Molecular Weight:369.24

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

α-NETA Chemical Structure

CAS. Nr.: 115066-04-1

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (13)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

ALDH1

AChE

Alle Chemerin Receptor Isoform-spezifische Produkte anzeigen :

ChemR23/CMKLR1

Alle Aldehyde Dehydrogenase (ALDH) Isoform-spezifische Produkte anzeigen :

Alle Cholinesterase (ChE) Isoform-spezifische Produkte anzeigen :

AChE
BChE

In Vitro

α-NETA (50-150 nM; 24 hours) decreases all cell lines viability in a dose-dependent manner^[3].
α-NETA (2.5-10.0 μg/mL; 24 hours) leads to epithelial ovarian cancer (EOC) cell death associated with membrane blistering and cytoplasm leakage^[3].
α-NETA treatment increases EOC cell expression of pyroptosis-associated proteins^[3].
α-NETA is most potent in inhibiting aldehyde dehydrogenase 1 family, member A1 (ALDH1A1; IC₅₀=0.04 μM; purified enzymes assay), followed by CMKLR1 (IC₅₀=0.375 μM for β-ARR2 recruitment; Cell-based assay) and G9a histone lysine methyltransferase (IC₅₀=0.50 μM; purified enzymes assay). α-NETA selectively inhibits chemerin-stimulated CMKLR1 association with β-arrestin2^[2].
α-NETA possesses fluorescent characteristics (excitation spectrum: maxima 255 and 297 nm; emission spectrum: maximum 437 nm) of naphthyl moiety^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:

Ho8910, Ho8910PM, A2780, and Iose80 cells
Concentration:

50, 100, 150 nM
Incubation Time:

24 hours
Result:

Decreased all cell lines viability in a dose-dependent manner.

Apoptosis Analysis^[3]

Cell Line:

Epithelial ovarian cancer (EOC) cell
Concentration:

2.5, 7.5, 10.0 µg/mL
Incubation Time:

24 hours
Result:

Led to EOC cell death associated with membrane blistering and cytoplasm leakage.

In Vivo

α-NETA (i.p.; 0.125 mg/kg; once every other day for 20 days) significantly decreases tumor volume and tumor weight^[3].
α-NETA (s.c. injection; 3 mg/kg or 10 mg/kg; daily; for 30 days) significantly delays the onset of EAE with 3 mg/kg, and completely suppresses clinical signs for an average of nine days with 10 mg/kg beyond the first appearance of disease in control female C57BL/6 mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

BALB/c nude mice with skov3 cells^[3]
Dosage:

0.125 mg/kg
Administration:

IP; once every other day for 20 days
Result:

Significantly decreased tumor volume and tumor weight.

Chemical Information

CAS. Nr. 115066-04-1
Appearance Solid
Molecular Weight 369.24
Formel C₁₆H₂₀INO
Color White to off-white
SMILES

O=C(CC[N+](C)(C)C)C1=CC=CC2=C1C=CC=C2.[I-]
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (13)

All Cell Imaging/StainingELISABio/Physico-chemical AssayFlow CytometryWBIn Vivo Efficacy StudyRT-PCR

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Journal Impact Factor

MedComm (2020)

Statins, but not proprotein convertase subtilisin-kexin type 9 inhibitors, lower chemerin in hypercholesterolemia via low-density lipoprotein receptor upregulation. [Abstract]

2024 Aug 31;5(9):e681. PMID: 39220103

α-NETA purchased from MedChemExpress. Usage Cited in: MedComm (2020). 2024 Aug 31;5(9):e681. [Abstract]

The CMKLR1 antagonist α-NETA, pravastatin, atorvastatin, rosuvastatin, alirocumab, and evolocumab prevented this effect in the presence of serum, while only α-NETA (3 µmol/L) and pravastatin prevented it in the presence of ApoA-I.

α-NETA purchased from MedChemExpress. Usage Cited in: MedComm (2020). 2024 Aug 31;5(9):e681. [Abstract]

Western blot analysis of cell lysates revealed that α‐NETA (3 µmol/L), either with or without chemerin‐9, upregulated HMGCR, ABCA1, and ABCG1, while a similar tendency was shown for liver X receptors α and β (LXRα and LXRβ).
Mol Ther

PAD4 promotes macrophage migration to aggravate tubulointerstitial injury in diabetic kidney disease. [Abstract]

2025 Oct 6:S1525-0016(25)00830-5. PMID: 41054305
Allergy

Acetylcholine From Solitary Chemosensory Cell, Not Neuron, Regulates Basal Cell Fate Driving Eosinophilic Chronic Rhinosinusitis With Nasal Polyps. [Abstract]

2025 Sep 15. PMID: 40951952

α-NETA purchased from MedChemExpress. Usage Cited in: Allergy. 2025 Sep 15. [Abstract]

Histogram showing Ach concentrations in the basolateral medium of ALI cultures under the following conditions: Untreated, α-NETA-treated, and IL-4/IL-5/IL-13 treatment with or without α-NETA (10 μM, basal media was changed every 2 days for 21 days), as measured by ELISA.
Acta Biomater

Vascular stent with immobilized anti-inflammatory chemerin 15 peptides mitigates neointimal hyperplasia and accelerates vascular healing. [Abstract]

2024 Apr 15:179:371-384. PMID: 38382829
Hypertension

Statins Prevent the Deleterious Consequences of Placental Chemerin Upregulation in Preeclampsia. [Abstract]

2024 Apr;81(4):861-875. PMID: 38361240

α-NETA purchased from MedChemExpress. Usage Cited in: Hypertension. 2024 Apr;81(4):861-875. [Abstract]

Chemerin-9 constricted both healthy and preeclamptic chorionic plate arteries although its effects were much smaller (P<0.0001) in the latter. In both groups, pravastatin (20 μmol/L) and the chemerin1/CMKLR1 receptor antagonist α-NETA (3 μmol/L) blocked these constrictor effects. The ET-1-induced constrictor effects were also smaller (P<0.01) in preeclamptic chorionic plate arteries.
Neural Regen Res

Chemerin 15 peptide reduces neuroinflammation via the ChemR23 receptor after ischemia-reperfusion injury. [Abstract]

2024 Sep 6. PMID: 39248165
Eur J Pharmacol

Chemerin-9 in paraventricular nucleus increases sympathetic outflow and blood pressure via glutamate receptor-mediated ROS generation. [Abstract]

2022 Dec 5:936:175343. PMID: 36306926
iScience

Chemerin 15 enhances microglial phagocytosis to attenuate cerebral ischemia-reperfusion injury through the ChemR23/p38 MAPK pathway. [Abstract]

2025 Aug 19;28(9):113396. PMID: 40970195

α-NETA purchased from MedChemExpress. Usage Cited in: iScience. 2025 Aug 19;28(9):113396. [Abstract]

α-NETA (100 nM, 24 h), a competitive inhibitor of ChemR23, was used in subsequent experiments. Compared with the OGD+C15 group, the proportion of microglia containing Zymosan and the Zymosan+ area decreased in the α-NETA group, showing no statistical difference from the OGD group.

α-NETA purchased from MedChemExpress. Usage Cited in: iScience. 2025 Aug 19;28(9):113396. [Abstract]

C15 administration downregulated ChemR23 expression, while a slight increase in its expression was observed in the OGD+C15+α-NETA (100 nM, 24 h) group compared with the OGD+C15 group.

α-NETA purchased from MedChemExpress. Usage Cited in: iScience. 2025 Aug 19;28(9):113396. [Abstract]

α-NETA (10 mg/kg) treatment increased HMGB1 levels. Western blot was used to measure the protein expression of HMGB1 after I/R in mice.
BMC Endocr Disord

α-NETA down-regulates CMKLR1 mRNA expression in ileum and prevents body weight gains collaborating with ERK inhibitor PD98059 in turn to alleviate hepatic steatosis in HFD-induced obese mice but no impact on ileal mucosal integrity and steatohepatitis progression. [Abstract]

2023 Jan 10;23(1):9. PMID: 36624417

α-NETA purchased from MedChemExpress. Usage Cited in: BMC Endocr Disord. 2023 Jan 10;23(1):9. [Abstract]

α‑NETA (30 mg/kg; gavage; single daily for 3 weeks) down‑regulates elevated mRNA expressions of CMKLR1 in ileal tissues of wild-type adult C57BL/6 mice (male, 8-10 weeks old, 18-22 g). normal diet group (ND), high-fat diet group (HFD), HFD+α-NETA group (NETA), HFD+PD98059 group (PD) and HFD+α-NETA+PD98059 group (NETA+PD).
Blood Press

Endothelin-1- and acetylcholine-mediated effects in human and rat vessels: impact of perivascular adipose tissue, diabetes, angiotensin II, and chemerin. [Abstract]

2024 Dec;33(1):2414072. PMID: 39387176
Eur J Neurosci

Chemerin in caudal division of nucleus tractus solitarius increases sympathetic activity and blood pressure. [Abstract]

2024 Sep;60(5):4830-4842. PMID: 39044301
Heliyon

Electroacupuncture ameliorates gastrointestinal dysfunction by modulating DMV cholinergic efferent signals to drive the vagus nerve in p-MCAO rats. [Abstract]

2024 Apr 9;10(8):e29426. PMID: 38638995
Research Square Preprint

2023 Sep 18.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 83.33 mg/mL (225.68 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.7083 mL

13.5413 mL

27.0827 mL

5 mM

0.5417 mL

2.7083 mL

5.4165 mL

10 mM

0.2708 mL

1.3541 mL

2.7083 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (5.63 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (5.63 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (5.63 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (281 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Sastry BV, et al. Relationships between chemical structure and inhibition of choline acetyltransferase by 2-(alpha-naphthoyl)ethyltrimethylammonium and related compounds. Pharmacol Res Commun. 1988 Sep;20(9):751-71. [Content Brief]

[2]. Graham KL, et al. A novel CMKLR1 small molecule antagonist suppresses CNS autoimmune inflammatory disease. PLoS One. 2014 Dec 1;9(12):e112925. [Content Brief]

[3]. Qiao L, et al. α-NETA induces pyroptosis of epithelial ovarian cancer cells through the GSDMD/caspase-4 pathway. FASEB J. 2019 Nov;33(11):12760-12767. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.7083 mL	13.5413 mL	27.0827 mL	67.7066 mL
	5 mM	0.5417 mL	2.7083 mL	5.4165 mL	13.5413 mL
	10 mM	0.2708 mL	1.3541 mL	2.7083 mL	6.7707 mL
	15 mM	0.1806 mL	0.9028 mL	1.8055 mL	4.5138 mL
	20 mM	0.1354 mL	0.6771 mL	1.3541 mL	3.3853 mL
	25 mM	0.1083 mL	0.5417 mL	1.0833 mL	2.7083 mL
	30 mM	0.0903 mL	0.4514 mL	0.9028 mL	2.2569 mL
	40 mM	0.0677 mL	0.3385 mL	0.6771 mL	1.6927 mL
	50 mM	0.0542 mL	0.2708 mL	0.5417 mL	1.3541 mL
	60 mM	0.0451 mL	0.2257 mL	0.4514 mL	1.1284 mL
	80 mM	0.0339 mL	0.1693 mL	0.3385 mL	0.8463 mL
	100 mM	0.0271 mL	0.1354 mL	0.2708 mL	0.6771 mL

Bioz

α-NETA

13 Cited Publications

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α-NETA

Publications Citing Use of MedChemExpress (MCE) α-NETA

Alle Chemerin Receptor Isoform-spezifische Produkte anzeigen

Alle Aldehyde Dehydrogenase (ALDH) Isoform-spezifische Produkte anzeigen

Alle Cholinesterase (ChE) Isoform-spezifische Produkte anzeigen