X-Neu5Ac sodium
Based on 1 Customer Validation
X-Neu5Ac (sodium) is a substrate for chromogenic assay of neuraminidase activity in bacterial expression systems; with a Km of 0.89 mM for neuraminidase.
For research use only. We do not sell to patients.
- Purity: 99.98%
- CAS No.: 160369-85-7
- Formula: C19H21BrClN2NaO9
- Molecular Weight:559.72
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Km: 0.89 mM (neuraminidase)[1]
X-Neu5Ac (sodium) is a good substrate (Km=0.89 mM) for neuraminidase and is quite stable under identical conditions in the absence of enzyme[1]. Staining with X-Neu5Ac and FRV LB is specific for sialidase and useful for quantitative analysis of sialidase activities[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 160369-85-7
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Appearance Solid
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Molecular Weight 559.72
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Formula C19H21BrClN2NaO9
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Color White to light yellow
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SMILES
OC[C@@H](O)[C@@H](O)[C@H]1[C@H](NC(C)=O)[C@@H](O)C[C@](OC2=CNC3=C2C(Cl)=C(Br)C=C3)(C(O[Na])=O)O1
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Synonyms
X-NeuNAc
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
H2O : 100 mg/mL (178.66 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (278 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Fujii I, et al. X-Neu5Ac: a novel substrate for chromogenic assay of neuraminidase activity in bacterial expression systems. Bioorg Med Chem. 1993 Aug;1(2):147-9. [Content Brief]
[2]. Minami A, et al. Imaging of sialidase activity in rat brain sections by a highly sensitive fluorescent histochemical method. Neuroimage. 2011 Sep 1;58(1):34-40. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.7866 mL | 8.9330 mL | 17.8661 mL | 44.6652 mL |
| 5 mM | 0.3573 mL | 1.7866 mL | 3.5732 mL | 8.9330 mL | |
| 10 mM | 0.1787 mL | 0.8933 mL | 1.7866 mL | 4.4665 mL | |
| 15 mM | 0.1191 mL | 0.5955 mL | 1.1911 mL | 2.9777 mL | |
| 20 mM | 0.0893 mL | 0.4467 mL | 0.8933 mL | 2.2333 mL | |
| 25 mM | 0.0715 mL | 0.3573 mL | 0.7146 mL | 1.7866 mL | |
| 30 mM | 0.0596 mL | 0.2978 mL | 0.5955 mL | 1.4888 mL | |
| 40 mM | 0.0447 mL | 0.2233 mL | 0.4467 mL | 1.1166 mL | |
| 50 mM | 0.0357 mL | 0.1787 mL | 0.3573 mL | 0.8933 mL | |
| 60 mM | 0.0298 mL | 0.1489 mL | 0.2978 mL | 0.7444 mL | |
| 80 mM | 0.0223 mL | 0.1117 mL | 0.2233 mL | 0.5583 mL | |
| 100 mM | 0.0179 mL | 0.0893 mL | 0.1787 mL | 0.4467 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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