Flubi-2
Flubi-2 is a fluorescein-biotin based pH-ratio dye (Ex=480 nm, Em= 520-560 nm) with a pK value of 6.7. Flubi-2 is a hydrolysis product of Flubida-2 (non-fluorescent, membrane permeable). Flubi-2 can be used for pH determination of organelles of the secretory pathway (such as golgi apparatus and endoplasmic reticulum) in living cells.
For research use only. We do not sell to patients.
- Formula: C78H88N8O16S2
- Molecular Weight:728.85
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
517
492
Chemical Information
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Molecular Weight 728.85
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Formula C78H88N8O16S2
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SMILES
O=C1C=CC(C(OC2=CC(O)=CC=C23)=C1)=C3C4=CC=C(C(N(C)CCCCCCN(C)C(CCCC[C@@]5([H])C(NC(N6)=O)C6CS5)=O)=O)C=C4C(O)=O.O=C7C=CC(C(OC8=CC(O)=CC=C89)=C7)=C9C%10=CC(C(N(C)CCCCCCN(C)C(CCCC[C@@]%11([H])C(NC(N%12)=O)C%12CS%11)=O)=O)=CC=C%10C(O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Wu MM, et al. Studying organelle physiology with fusion protein-targeted avidin and fluorescent biotin conjugates. Methods Enzymol. 2000;327:546-64. [Content Brief]
[2]. Wu MM, et al. Organelle pH studies using targeted avidin and fluorescein-biotin. Chem Biol. 2000 Mar;7(3):197-209. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)