Azide-A-DSBSO crosslinker
Based on 1 Customer Validation
Azide-A-DSBSO crosslinker is a mass spectrometry (MS) cleavable, membrane permeable, homobifunctional, azide-labeled, acid-cleavable crosslinking peptide. Azide-A-DSBSO crosslinker crosslinks proteins via NHS ester reaction with lysine residues. Azide-A-DSBSO crosslinker can be used to study protein-protein interactions by cross-linking mass spectrometry (XL-MS) [1].
For research use only. We do not sell to patients.
- Purity: 95.0%
- CAS No.: 1704097-02-8
- Formula: C24H33N5O12S2
- Molecular Weight:647.68
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
DSBSO linker (1 mM; 1 h) is used in cross-linking experiments with recombinant Cas9 protein and E. coli ribosome[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1704097-02-8
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Appearance Solid
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Molecular Weight 647.68
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Formula C24H33N5O12S2
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Color White to off-white
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SMILES
[N-]=[N+]=NCCCC1(C)OCC(CS(CCC(ON2C(CCC2=O)=O)=O)=O)(CS(CCC(ON3C(CCC3=O)=O)=O)=O)CO1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 100 mg/mL (154.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Matzinger M, et al. Fast and Highly Efficient Affinity Enrichment of Azide-A-DSBSO Cross-Linked Peptides. J Proteome Res. 2020 May 1;19(5):2071-2079. [Content Brief]
[2]. Burke AM, et al. Synthesis of two new enrichable and MS-cleavable cross-linkers to define protein-protein interactions by mass spectrometry. Org Biomol Chem. 2015 May 7;13(17):5030-7. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5440 mL | 7.7199 mL | 15.4397 mL | 38.5993 mL |
| 5 mM | 0.3088 mL | 1.5440 mL | 3.0879 mL | 7.7199 mL | |
| 10 mM | 0.1544 mL | 0.7720 mL | 1.5440 mL | 3.8599 mL | |
| 15 mM | 0.1029 mL | 0.5147 mL | 1.0293 mL | 2.5733 mL | |
| 20 mM | 0.0772 mL | 0.3860 mL | 0.7720 mL | 1.9300 mL | |
| 25 mM | 0.0618 mL | 0.3088 mL | 0.6176 mL | 1.5440 mL | |
| 30 mM | 0.0515 mL | 0.2573 mL | 0.5147 mL | 1.2866 mL | |
| 40 mM | 0.0386 mL | 0.1930 mL | 0.3860 mL | 0.9650 mL | |
| 50 mM | 0.0309 mL | 0.1544 mL | 0.3088 mL | 0.7720 mL | |
| 60 mM | 0.0257 mL | 0.1287 mL | 0.2573 mL | 0.6433 mL | |
| 80 mM | 0.0193 mL | 0.0965 mL | 0.1930 mL | 0.4825 mL | |
| 100 mM | 0.0154 mL | 0.0772 mL | 0.1544 mL | 0.3860 mL |
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