Azide-A-DSBSO crosslinker
Based on 1 Customer Validation
Azide-A-DSBSO crosslinker is a mass spectrometry (MS) cleavable, membrane permeable, homobifunctional, azide-labeled, acid-cleavable crosslinking peptide. Azide-A-DSBSO crosslinker crosslinks proteins via NHS ester reaction with lysine residues. Azide-A-DSBSO crosslinker can be used to study protein-protein interactions by cross-linking mass spectrometry (XL-MS) [1].
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 95.0%
- CAS 番号: 1704097-02-8
- 分子式: C24H33N5O12S2
- 分子量:647.68
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保管条件:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
体外実験
DSBSO linker (1 mM; 1 h) is used in cross-linking experiments with recombinant Cas9 protein and E. coli ribosome[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 1704097-02-8
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性状 Solid
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分子量 647.68
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分子式 C24H33N5O12S2
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Color White to off-white
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SMILES
[N-]=[N+]=NCCCC1(C)OCC(CS(CCC(ON2C(CCC2=O)=O)=O)=O)(CS(CCC(ON3C(CCC3=O)=O)=O)=O)CO1
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶剤 & 溶解度
体外:
DMSO : 100 mg/mL (154.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Matzinger M, et al. Fast and Highly Efficient Affinity Enrichment of Azide-A-DSBSO Cross-Linked Peptides. J Proteome Res. 2020 May 1;19(5):2071-2079. [Content Brief]
[2]. Burke AM, et al. Synthesis of two new enrichable and MS-cleavable cross-linkers to define protein-protein interactions by mass spectrometry. Org Biomol Chem. 2015 May 7;13(17):5030-7. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5440 mL | 7.7199 mL | 15.4397 mL | 38.5993 mL |
| 5 mM | 0.3088 mL | 1.5440 mL | 3.0879 mL | 7.7199 mL | |
| 10 mM | 0.1544 mL | 0.7720 mL | 1.5440 mL | 3.8599 mL | |
| 15 mM | 0.1029 mL | 0.5147 mL | 1.0293 mL | 2.5733 mL | |
| 20 mM | 0.0772 mL | 0.3860 mL | 0.7720 mL | 1.9300 mL | |
| 25 mM | 0.0618 mL | 0.3088 mL | 0.6176 mL | 1.5440 mL | |
| 30 mM | 0.0515 mL | 0.2573 mL | 0.5147 mL | 1.2866 mL | |
| 40 mM | 0.0386 mL | 0.1930 mL | 0.3860 mL | 0.9650 mL | |
| 50 mM | 0.0309 mL | 0.1544 mL | 0.3088 mL | 0.7720 mL | |
| 60 mM | 0.0257 mL | 0.1287 mL | 0.2573 mL | 0.6433 mL | |
| 80 mM | 0.0193 mL | 0.0965 mL | 0.1930 mL | 0.4825 mL | |
| 100 mM | 0.0154 mL | 0.0772 mL | 0.1544 mL | 0.3860 mL |
Azide-A-DSBSO crosslinker 関連分類
HY-157414 関連分類
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