Azide-PEG10000-OPSS
Azide-PEG10000-OPSS (N3-PEG10000-OPSS) is a compound composed of an azide group, PEG, and o-dithiopyridine (OPSS) and can be used for drug delivery. Azide-PEG10000-OPSS is a click chemistry reagent. It contains an Azide group and can undergo a copper-catalyzed azido-yne cycloaddition reaction (CuAAc) with molecules containing an Alkyne group. It can also undergo ring-strain-driven acetylene-azidocycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
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- 분자량:10000 (Average)
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보관:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocol
- 순도&문서
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Help & FAQs
Biological Activity
제품 설명
Chemical Information
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분자량 10000 (Average)
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SMILES
O=C(NCCOCCN=[N+]=[N-])CCSSC1=NC=CC=C1.[n]
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Synonyms
N3-PEG10000-OPSS
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocol
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
순도&문서
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Azide-PEG10000-OPSS Related Classifications
HY-179193H Related Classifications
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