Azido-Bodipy-FL-510
Azido-Bodipy-FL-510 is a derivative of BODIPY-FL-510 containing an azide group, designed for use in click chemistry reactions (CuAAC). It is primarily used for click chemistry-based bioconjugation (Ex/Em = 510/520 nm).
For research use only. We do not sell to patients.
- CAS No.: 1048369-35-2
- Formula: C29H34BF2N7O7
- Molecular Weight:641.43
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
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Help & FAQs
Biological Activity
Description
Chemical Information
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CAS No. 1048369-35-2
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Molecular Weight 641.43
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Formula C29H34BF2N7O7
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SMILES
[N-]=[N+]=NC(COC1=CC=CC(C(NCCNC(CCC2=CC=C3C=C4C(C)=CC(C)=[N]4[B+3]([F-])([N-]32)[F-])=O)=O)=C1)OCCOCC([O-])=O.[H+]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Azido-Bodipy-FL-510 Related Classifications
HY-W998656 Related Classifications
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