Biotin-C1-PEG3-C3-amido-C5-Gly-Arg-Gly-N3 TFA
Based on 1 Customer Validation
Biotin-C1-PEG3-C3-amido-C5-Gly-Arg-Gly-N3 TFA is used for detection of modification site for N-myristoylated and GPI-anchored proteins in blood-stage P. falciparum. Biotin-C1-PEG3-C3-amido-C5-Gly-Arg-Gly-N3 (TFA) is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
For research use only. We do not sell to patients.
- Purity : 98.0%
- Formula: C38H66F3N13O11S
- Molecular Weight:970.07
-
Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Protocols
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Description
Chemical Information
-
Appearance Solid
-
Molecular Weight 970.07
-
Formula C38H66F3N13O11S
-
Color White to off-white
-
SMILES
[N-]=[N+]=NCC(N[C@@H](CCCNC(N)=N)C(NCC(NCCCCCC(NCCCOCCOCCOCCCNC(CCCC[C@H]1[C@]2([H])[C@](NC(N2)=O)([H])CS1)=O)=O)=O)=O)=O.OC(C(F)(F)F)=O
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (103.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
-
EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
-
Data Sheet (274 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.0309 mL | 5.1543 mL | 10.3085 mL | 25.7713 mL |
| 5 mM | 0.2062 mL | 1.0309 mL | 2.0617 mL | 5.1543 mL | |
| 10 mM | 0.1031 mL | 0.5154 mL | 1.0309 mL | 2.5771 mL | |
| 15 mM | 0.0687 mL | 0.3436 mL | 0.6872 mL | 1.7181 mL | |
| 20 mM | 0.0515 mL | 0.2577 mL | 0.5154 mL | 1.2886 mL | |
| 25 mM | 0.0412 mL | 0.2062 mL | 0.4123 mL | 1.0309 mL | |
| 30 mM | 0.0344 mL | 0.1718 mL | 0.3436 mL | 0.8590 mL | |
| 40 mM | 0.0258 mL | 0.1289 mL | 0.2577 mL | 0.6443 mL | |
| 50 mM | 0.0206 mL | 0.1031 mL | 0.2062 mL | 0.5154 mL | |
| 60 mM | 0.0172 mL | 0.0859 mL | 0.1718 mL | 0.4295 mL | |
| 80 mM | 0.0129 mL | 0.0644 mL | 0.1289 mL | 0.3221 mL | |
| 100 mM | 0.0103 mL | 0.0515 mL | 0.1031 mL | 0.2577 mL |
Biotin-C1-PEG3-C3-amido-C5-Gly-Arg-Gly-N3 TFA Related Classifications
HY-131455A Related Classifications
Powered by Bioz