Boc-Phe-Ser-Arg-AMC TFA
Based on 1 Customer Validation
Boc-Phe-Ser-Arg-AMC TFA is a trypsin peptide substrate containing a Boc protecting group. Boc-Phe-Ser-Arg-AMC TFA can be degraded by trypsin, releasing AMC (HY-D0027) which has fluorescence activity. Boc-Phe-Ser-Arg-AMC TFA can be used to assess the activity of trypsin.
For research use only. We do not sell to patients.
- Formula: C35H44F3N7O10
- Molecular Weight:779.76
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Biological Activity
Chemical Information
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Appearance Solid
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Molecular Weight 779.76
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Formula C35H44F3N7O10
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SMILES
CC(C1=CC=C(NC([C@H](CCCNC(N)=N)NC([C@H](CO)NC([C@@H](NC(OC(C)(C)C)=O)CC2=CC=CC=C2)=O)=O)=O)C=C1O3)=CC3=O.OC(C(F)(F)F)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (128.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2824 mL | 6.4122 mL | 12.8245 mL | 32.0611 mL |
| 5 mM | 0.2565 mL | 1.2824 mL | 2.5649 mL | 6.4122 mL | |
| 10 mM | 0.1282 mL | 0.6412 mL | 1.2824 mL | 3.2061 mL | |
| 15 mM | 0.0855 mL | 0.4275 mL | 0.8550 mL | 2.1374 mL | |
| 20 mM | 0.0641 mL | 0.3206 mL | 0.6412 mL | 1.6031 mL | |
| 25 mM | 0.0513 mL | 0.2565 mL | 0.5130 mL | 1.2824 mL | |
| 30 mM | 0.0427 mL | 0.2137 mL | 0.4275 mL | 1.0687 mL | |
| 40 mM | 0.0321 mL | 0.1603 mL | 0.3206 mL | 0.8015 mL | |
| 50 mM | 0.0256 mL | 0.1282 mL | 0.2565 mL | 0.6412 mL | |
| 60 mM | 0.0214 mL | 0.1069 mL | 0.2137 mL | 0.5344 mL | |
| 80 mM | 0.0160 mL | 0.0802 mL | 0.1603 mL | 0.4008 mL | |
| 100 mM | 0.0128 mL | 0.0641 mL | 0.1282 mL | 0.3206 mL |