2. Metabolic Enzyme/Protease
  3. Ser/Thr Protease
  4. Boc-Phe-Ser-Arg-AMC TFA

Boc-Phe-Ser-Arg-AMC TFA is a trypsin peptide substrate containing a Boc protecting group. Boc-Phe-Ser-Arg-AMC TFA can be degraded by trypsin, releasing AMC (HY-D0027) which has fluorescence activity. Boc-Phe-Ser-Arg-AMC TFA can be used to assess the activity of trypsin.

For research use only. We do not sell to patients.

Boc-Phe-Ser-Arg-AMC TFA

Boc-Phe-Ser-Arg-AMC TFA Chemical Structure

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Other Forms of Boc-Phe-Ser-Arg-AMC TFA:

Boc-Phe-Ser-Arg-AMC TFA Related Antibodies

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Description

Boc-Phe-Ser-Arg-AMC TFA is a trypsin peptide substrate containing a Boc protecting group. Boc-Phe-Ser-Arg-AMC TFA can be degraded by trypsin, releasing AMC (HY-D0027) which has fluorescence activity. Boc-Phe-Ser-Arg-AMC TFA can be used to assess the activity of trypsin[1].

Molecular Weight

779.76

Formula

C35H44F3N7O10
Appearance

Solid

SMILES

CC(C1=CC=C(NC([C@H](CCCNC(N)=N)NC([C@H](CO)NC([C@@H](NC(OC(C)(C)C)=O)CC2=CC=CC=C2)=O)=O)=O)C=C1O3)=CC3=O.OC(C(F)(F)F)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (128.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.2824 mL 6.4122 mL 12.8245 mL
5 mM 0.2565 mL 1.2824 mL 2.5649 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
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g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.2824 mL 6.4122 mL 12.8245 mL 32.0611 mL
5 mM 0.2565 mL 1.2824 mL 2.5649 mL 6.4122 mL
10 mM 0.1282 mL 0.6412 mL 1.2824 mL 3.2061 mL
15 mM 0.0855 mL 0.4275 mL 0.8550 mL 2.1374 mL
20 mM 0.0641 mL 0.3206 mL 0.6412 mL 1.6031 mL
25 mM 0.0513 mL 0.2565 mL 0.5130 mL 1.2824 mL
30 mM 0.0427 mL 0.2137 mL 0.4275 mL 1.0687 mL
40 mM 0.0321 mL 0.1603 mL 0.3206 mL 0.8015 mL
50 mM 0.0256 mL 0.1282 mL 0.2565 mL 0.6412 mL
60 mM 0.0214 mL 0.1069 mL 0.2137 mL 0.5344 mL
80 mM 0.0160 mL 0.0802 mL 0.1603 mL 0.4008 mL
100 mM 0.0128 mL 0.0641 mL 0.1282 mL 0.3206 mL
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Boc-Phe-Ser-Arg-AMC TFA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Boc-Phe-Ser-Arg-AMCSer/Thr ProteaseSerine proteasesSerine endopeptidasesThreonine proteasesInhibitorinhibitorinhibit

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