BODIPY 505/515-8-C3-COOH

Drug screening technologies are experimental and computational strategies used to identify small molecules or chemical probes that modulate a defined molecular target, signaling pathway, cellular phenotype, disease model, or patient-derived response profile. High-throughput screening tests many compounds in miniaturized assay formats, while quantitative high-throughput screening tests compounds across concentration ranges so that potency and efficacy can be inferred from concentration-response behavior rather than from a single-point signal. The core biological function of a drug-screening strategy is to connect compound exposure with measurable pathway activity, target modulation, cell-state change, viability, cytotoxicity, morphology, or disease-relevant phenotype. Assay performance must be evaluated before screening because hit identification depends on the separation between positive and negative controls, control variability, plate effects, outliers, and the statistical framework

Flow cytometry (FC) is a technique for high-speed, step-by-step quantitative analysis and sorting of single cells or other biological particles in a suspension by detecting labeled fluorescent signals.

Fluorescence activated cell sorting (FACS) is a special technology for identifying and separating rare cell populations with high purity.

Fluorescence-Activated Cell Sorting (FACS) separates cells in suspension after flow-cytometric measurement of light scatter and fluorescence; classic droplet-based instruments interrogate cells with a laser, convert optical signals into electronic signals, charge droplets containing target cells, and electrostatically deflect them into collection vessels. FACS detects phenotypes defined by fluorescent antibodies, fluorescent proteins, viability dyes, or intracellular markers, and the readout is the sorted fraction, purity, recovery, and post-sort viability of the gated population.

Lipid droplets are intracellular organelles with a neutral-lipid core that stores triacylglycerols and sterol esters, and Oil Red O or Sudan dyes detect these hydrophobic lipid deposits by partitioning into retained lipids in fresh or frozen specimens. Oil Red O stains neutral triglycerides and lipids in frozen tissue sections or air-dried cytologic preparations, while Sudan Black B has also been used as a histochemical fat stain for lipid-rich tissue structures.