2. Others Apoptosis
  3. Fluorescent Dye Apoptosis
  4. BODIPY−DOX

BODIPY-DOX is a conjugate composed of BODIPY and Doxorubicin (HY-15142A), as well as a pH-activated fluorescent probe for M1 macrophages and an apoptosis inducer. BODIPY-DOX undergoes pH-induced hydrazone bond cleavage in acidic M1 macrophage phagosomes, thereby releasing cytotoxic Doxorubicin (Dox) and inhibiting the function of pro-inflammatory M1 macrophages. BODIPY-DOX highly selectively inhibits the production of relevant pro-inflammatory cytokines by mouse and human monocyte-derived M1 macrophages, while exerting minimal effects on M2 or unactivated macrophages. Therefore, BODIPY-DOX enables simultaneous fluorescent tracing, differentiation and elimination of specific macrophage subsets, and exhibits the potential to regulate tissue regeneration in zebrafish models.

For research use only. We do not sell to patients.

BODIPY−DOX

BODIPY−DOX Chemical Structure

CAS No. : 2173116-67-9

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

BODIPY−DOX Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

BODIPY-DOX is a conjugate composed of BODIPY and Doxorubicin (HY-15142A), as well as a pH-activated fluorescent probe for M1 macrophages and an apoptosis inducer. BODIPY-DOX undergoes pH-induced hydrazone bond cleavage in acidic M1 macrophage phagosomes, thereby releasing cytotoxic Doxorubicin (Dox) and inhibiting the function of pro-inflammatory M1 macrophages. BODIPY-DOX highly selectively inhibits the production of relevant pro-inflammatory cytokines by mouse and human monocyte-derived M1 macrophages, while exerting minimal effects on M2 or unactivated macrophages. Therefore, BODIPY-DOX enables simultaneous fluorescent tracing, differentiation and elimination of specific macrophage subsets, and exhibits the potential to regulate tissue regeneration in zebrafish models[1].

In Vitro

BODIPY-DOX (5-10 μM; overnight) induces dose-dependent cytotoxicity in LPS-polarized M1 RAW264.7 murine macrophages, but does not affect cell viability in nonactivated or IL-4-polarized M2 RAW264.7 murine macrophages[1].
BODIPY-DOX (10 μM; 20 h) activates to release fluorescent signal and induce apoptosis specifically in LPS-polarized M1 RAW264.7 murine macrophages, with minimal effects on nonactivated RAW264.7 murine macrophages[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

BODIPY−DOX Related Antibodies

Cell Viability Assay[1]

Cell Line: LPS-polarized M1 RAW264.7 murine macrophages, IL-4-polarized M2 RAW264.7 murine macrophages, nonactivated RAW264.7 murine macrophages
Concentration: 5 μM, 10 μM
Incubation Time: overnight
Result: Reduced normalized cell viability of M1 macrophages to ~0.8.
Reduced normalized cell viability of M1 macrophages to ~0.65.
Did not reduce normalized cell viability in nonactivated or M2 macrophages at either concentration.

Apoptosis Analysis[1]

Cell Line: LPS-polarized M1 RAW264.7 murine macrophages, nonactivated RAW264.7 murine macrophages
Concentration: 10 μM
Incubation Time: 20 h
Result: Showed strong BODIPY fluorescence staining in LPS-polarized M1 macrophages.
Increased apoptotic index (Annexin V-positive cells) of LPS-polarized M1 macrophages to 3-fold higher than that of nonactivated macrophages.
Exhibited coincident strong BODIPY fluorescence in apoptotic M1 macrophages.
Showed low BODIPY fluorescence staining and a low apoptotic index in nonactivated macrophages.
Molecular Weight

1049.92

Formula

C54H62BF2N7O12
CAS No.
SMILES

[H][C@@]1(C[C@](/C(CO)=N\NC(CCC2=[N+]3C(C(C)=C2)=C(C4=C(C=C(N4[B-]3(F)F)C)C)C5=CC=C(C=C5)NC(CN(CC)CC)=O)=O)(CC6=C(C7=C(C(O)=C61)C(C8=C(C=CC=C8C7=O)OC)=O)O)O)O[C@H]9C[C@@H]([C@@H]([C@@H](O9)C)O)N
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

BODIPY−DOX Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BODIPY−DOX2173116-67-9Fluorescent DyeApoptosisproinflammatory cytokineapoptosishydrazone linkerdoxorubicinM1 macrophagehuman monocyte-derived M1 macrophagesRAW264.7 murine macrophageszebrafish modelmurine monocyte-derived M1 macrophagesphagosomesInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
BODIPY−DOX
Cat. No.:
HY-D3190
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.