Bodipy TMR-X muscimol
Based on 1 Customer Validation
Bodipy TMR-X muscimol is a Bodipy labeled Muscimol (HY-N2313) (Ex=543 nm, Em=572 nm). Muscimol is a GABAA agonist. Bodipy TMR-X muscimol can be used for imaging the spread of reversible brain inactivations.
For research use only. We do not sell to patients.
- Purity: 99.79%
- CAS No.: 849464-08-0
- Formula: C31H36BF2N5O5
- Molecular Weight:607.46
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
GABAA receptor[1]
1. Rats are infused with Bodipy TMR-X muscimol as below: dilute the stock solution to 2 μg/μL (in PBS), infused into both hemispheres at a rate of 0.25 μL/min for a single minute, resulting in a final infusion volume of 0.25 μL and a final dose of 0.5 μg per side.
2. The animals are sacrificed by rapid decapitation 15 min after infusion in order to match the spread to what the experimental animals received immediately prior to behavioral testing.
3. The brains are removed and flash frozen in 45°C isopentane, stored at 80°C.
4. Coronal slices are sectioned at 60 μm. The slices are mounted on charged microscope slides and counterstained with DAPI.
5. The stained slices are incubated in a cool, dark room at room temperature for 3 d before being visualized with a confocal microscope.
6. A digital plate from the Paxinos and Watson (2007) rat brain atlas is overlaid on the image to visualize the spread.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
570
544
Chemical Information
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CAS No. 849464-08-0
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Appearance Solid
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Molecular Weight 607.46
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Formula C31H36BF2N5O5
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Color Brown to reddish brown
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SMILES
[F-][B+3]1([N-]2C(C=C(C(C)=C3CCC(NCCCCCC(NCC4=CC(O)=NO4)=O)=O)[N]1=C3C)=CC=C2C5=CC=C(OC)C=C5)[F-]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (164.62 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (271 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Timothy A Allen, et al. Imaging the spread of reversible brain inactivations using fluorescent muscimol. J Neurosci Methods. 2008 Jun 15;171(1):30-8. [Content Brief]
[2]. Nicholas A Heroux, et al. Differential involvement of the medial prefrontal cortex across variants of contextual fear conditioning. Learn Mem. 2017 Jul 17;24(8):322-330. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.6462 mL | 8.2310 mL | 16.4620 mL | 41.1550 mL |
| 5 mM | 0.3292 mL | 1.6462 mL | 3.2924 mL | 8.2310 mL | |
| 10 mM | 0.1646 mL | 0.8231 mL | 1.6462 mL | 4.1155 mL | |
| 15 mM | 0.1097 mL | 0.5487 mL | 1.0975 mL | 2.7437 mL | |
| 20 mM | 0.0823 mL | 0.4115 mL | 0.8231 mL | 2.0577 mL | |
| 25 mM | 0.0658 mL | 0.3292 mL | 0.6585 mL | 1.6462 mL | |
| 30 mM | 0.0549 mL | 0.2744 mL | 0.5487 mL | 1.3718 mL | |
| 40 mM | 0.0412 mL | 0.2058 mL | 0.4115 mL | 1.0289 mL | |
| 50 mM | 0.0329 mL | 0.1646 mL | 0.3292 mL | 0.8231 mL | |
| 60 mM | 0.0274 mL | 0.1372 mL | 0.2744 mL | 0.6859 mL | |
| 80 mM | 0.0206 mL | 0.1029 mL | 0.2058 mL | 0.5144 mL | |
| 100 mM | 0.0165 mL | 0.0823 mL | 0.1646 mL | 0.4115 mL |
Bodipy TMR-X muscimol Related Classifications
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