BETA-1
Based on 1 Customer Validation
BETA-1 is the first twisted intramolecular charge transfer (TICT)-aggregation-induced emission (AIE) integration molecule. BETA-1 emits cyan fluorescence in lipid droplets (LDs) and red fluorescence in mitochondria. BETA-1 can be used for the simultaneous and dual-color imaging of LDs and mitochondria in vivo and in vitro.
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- Pureté : 99.42%
- CAS No.: 2924598-24-1
- Formule: C37H28IN3S2
- Masse moléculaire:705.67
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Stockage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
In Vitro
Guide (The following is our recommended solution. This solution is merely a guideline and should be modified according to your specific needs.)
The HeLa cells were cultured in DMEM medium supplemented with 10% FBS, in a 37 °C CO?/air (5%/95%) incubator.
2. 5.0 × 103 HeLa cells were inoculated into each well of a 96-well glass-bottom plate and incubated for 24 hours.
3. For cell imaging, live HeLa cells were stained with 5 μM BETA-1 at 37 °C for 30 minutes.
4. Collect the emission light of BETA-1 under the excitation wavelengths of 405 or 561 nm. Record digital images using a laser scanning confocal microscope. Analyze the images using Fiji/ImageJ.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Essai clinique
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 2924598-24-1
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Appearance Solid
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Masse moléculaire 705.67
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Formule C37H28IN3S2
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Color Brown to black
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SMILES
C[N+]1=C(/C=C/C2=CC=C(N(C3=CC=C(/C=C/C4=NC5=CC=CC=C5S4)C=C3)C6=CC=CC=C6)C=C2)SC7=CC=CC=C17.[I-]
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvant et solubilité
In Vitro:
DMSO : 25 mg/mL (35.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocole
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Lipid Droplets: Oil Red O/Sudan Dye Lipid Staining
Lipid droplets are intracellular organelles with a neutral-lipid core that stores triacylglycerols and sterol esters, and Oil Red O or Sudan dyes detect these hydrophobic lipid deposits by partitioning into retained lipids in fresh or frozen specimens. Oil Red O stains neutral triglycerides and lipids in frozen tissue sections or air-dried cytologic preparations, while Sudan Black B has also been used as a histochemical fat stain for lipid-rich tissue structures.
Pureté et documentation
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Fiche technique (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.4171 mL | 7.0855 mL | 14.1709 mL | 35.4273 mL |
| 5 mM | 0.2834 mL | 1.4171 mL | 2.8342 mL | 7.0855 mL | |
| 10 mM | 0.1417 mL | 0.7085 mL | 1.4171 mL | 3.5427 mL | |
| 15 mM | 0.0945 mL | 0.4724 mL | 0.9447 mL | 2.3618 mL | |
| 20 mM | 0.0709 mL | 0.3543 mL | 0.7085 mL | 1.7714 mL | |
| 25 mM | 0.0567 mL | 0.2834 mL | 0.5668 mL | 1.4171 mL | |
| 30 mM | 0.0472 mL | 0.2362 mL | 0.4724 mL | 1.1809 mL |
BETA-1 Related Classifications
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