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Cat. No.: HY-138894

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Nat 20(S)-yne

Nat 20(S)-yne is a steroid with an alkyne group at the terminal end, and it can be used in click chemistry reactions.

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CAS No.: 1397692-46-4
Formule: C₂₅H₃₈O₂
Masse moléculaire:370.57

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Please store the product under the recommended conditions in the Certificate of Analysis.

Nat 20(S)-yne Chemical Structure

CAS No.: 1397692-46-4

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique

Description

Nat 20(S)-yne is a steroid with an alkyne group at the terminal end, and it can be used in click chemistry reactions.

Chemical Information

CAS No. 1397692-46-4
Masse moléculaire 370.57
Formule C₂₅H₃₈O₂
SMILES

C[C@@]12[C@](CC[C@]2([H])[C@@](C)(O)CCC#C)([H])[C@@]3([H])[C@]([C@@]4(C(C[C@H](CC4)O)=CC3)C)([H])CC1
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

Please store the product under the recommended conditions in the Certificate of Analysis.

Protocole

All Cell Biology

EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.

Pureté et documentation

Instruction de manipulation (2659 KB)

Références

[1]. Faletrov YV, Efimova VS, Horetski MS, et al. New 20-hydroxycholesterol-like compounds with fluorescent NBD or alkyne labels: Synthesis, in silico interactions with proteins and uptake by yeast cells. Chem Phys Lipids. 2020;227:104850. [Content Brief]

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Calculateur de molarité

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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