SNAP-Biotin
SNAP-Biotin is a cell-permeable SNAP-tag-targeted labeling reagent. SNAP-Biotin undergoes an irreversible covalent reaction with SNAP-tag to transfer biotin to the tag. SNAP-Biotin enables the detection of SNAP-tag fusion proteins via a streptavidin-HRP immunoblotting system and achieves biotinylation of SNAP-tag fusion proteins. SNAP-Biotin is applicable to triple-negative breast cancer-related research.
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- CAS No.: 471918-16-8
- Formule: C29H39N9O4S
- Masse moléculaire:609.74
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Stockage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Activité biologique
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Chemical Information
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
Description
In Vitro
SNAP-Biotin (150 nM-1.5 mM) binds to the inactive SNAP-tag mutant SNAPC145A with measurable affinity[2].
SNAP-Biotin (1.5 μM; 30 min incubation, 11 h chase) labels parental H3.3-SNAP proteins in mouse embryonic stem cells[3].
SNAP-Biotin (1.5 μM; 30 min incubation, 14 h chase) labels parental H3.3-SNAP in mouse embryonic stem cells, where Mcm2-Pola1 and Pole3-Pole4 promote the transfer of parental H3.3-SNAP to the lagging strand and leading strand of DNA replication, respectively[3].
SNAP-Biotin (30 μM; 1 h) specifically labels the HCC1 fusion protein with a functional C-terminal SNAP tag in crude mitochondrial extracts from transgenic Arabidopsis hcc1-1 mutants[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 471918-16-8
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Appearance Solid
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Masse moléculaire 609.74
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Formule C29H39N9O4S
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Color Off-white to light yellow
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SMILES
NC1=NC(OCC(C=C2)=CC=C2CNC(CCCCCNC(CCCC[C@H]3[C@]4([H])[C@](NC(N4)=O)([H])CS3)=O)=O)=C5C(N=CN5)=N1
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Pureté et documentation
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Fiche technique (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Stoops EH, et al. SNAP-tag to monitor trafficking of membrane proteins in polarized epithelial cells. Methods in molecular biology (Clifton, N.J.). 2014;1174:171-82. [Content Brief]
[2]. Wilhelm J, et al. Kinetic and Structural Characterization of the Self-Labeling Protein Tags HaloTag7, SNAP-tag, and CLIP-tag. Biochemistry. 2021 Aug 24;60(33):2560-2575. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
SNAP-Biotin Related Classifications
HY-148958 Related Classifications
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