SOAT-IN-2
SOAT-IN-2 is a selective sodium-dependent organic anion transporter (SOAT, SLC10A6) inhibitor. SOAT-IN-2 blocks sodium-dependent cellular uptake of sulfated steroid Dehydroepiandrosterone sulfate (DHEAS, HY-113416). SOAT-IN-2 reduces intracellular estradiol synthesis, DHEA (HY-14650) formation, and DHEAS-stimulated cancer cell proliferation without inducing cytotoxicity. SOAT-IN-2 can be used for the research of breast cancer.
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- CAS No.: 3056727-95-5
- Formule: C19H12Cl3N3O5S
- Masse moléculaire:500.74
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
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- Pureté et documentation
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Activité biologique
Description
In Vitro
SOAT-IN-2 (compound 12) (10 μM; 5 min preincubation) potently inhibits sodium-dependent SOAT-mediated [3H]DHEAS uptake in MCF-7_SOAT cells[1].
SOAT-IN-2 (10 μM; 10 min preincubation, 3 days combined treatment with 10 μM DHEAS) completely suppresses DHEAS-stimulated proliferation in MCF-7_SOAT cells[1].
SOAT-IN-2 (10 μM; 30 min preincubation, 72 h combined treatment with 10 μM DHEAS) significantly reduces DHEAS-derived DHEA and estradiol formation in MCF-7_SOAT cells[1].
SOAT-IN-2 inhibits SOAT-mediated DHEAS transport in SOAT-transfected HEK293 cells with an IC50 of 1.9 μM[1].
SOAT-IN-2 (0.1-10 μM; 24 h) is non-cytotoxic to MCF-7_FRT and MCF-7_SOAT cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:SOAT-overexpressing MCF-7 breast cancer cells (MCF-7_SOAT)
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Concentration:10 μM
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Incubation Time:10 min
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Result:Had no effect on MCF-7_SOAT cell proliferation.
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Cell Line:SOAT-overexpressing MCF-7 breast cancer cells (MCF-7_SOAT)
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Concentration:10 μM plus 10 μM DEHA
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Incubation Time:3 days
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Result:Completely abolished the DHEAS-stimulated proliferation of MCF-7_SOAT cells, reducing proliferation to baseline control levels when combined with DHEAS.
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Cell Line:SOAT-overexpressing MCF-7 breast cancer cells (MCF-7_SOAT), control MCF-7_FRT cells
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Concentration:0.1 μM; 10 μM
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Incubation Time:24 h
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Result:Did not induce LDH release in either MCF-7_FRT or MCF-7_SOAT cells, indicating no cytotoxicity under these conditions.
- Mammalian live/dead viability and cytotoxicity staining
- BrdU Incorporation Assay
- Somatic Cell Culture
- CFSE Dye Dilution Proliferation Assay
- Dye-dilution cell tracking and proliferation staining
- CCK-8/WST-8 Cell Proliferation Assay
- Protocol for Cell Cycle
- Cell Counting-Based Growth Curve Assay
- Colony Formation (Clonogenic) Assay
- Breast Cancer Modeling
- Cell Viability Determination by MTT Colorimetric Assay
- EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
- Ki-67 Immunostaining Proliferation Assay
- PCNA Immunodetection Proliferation Assay
- Cell Cytotoxicity Assay
- Protocol for Cell Counting and Cell Density Analysis
- MTT Cell Proliferation Assay
Chemical Information
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CAS No. 3056727-95-5
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Masse moléculaire 500.74
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Formule C19H12Cl3N3O5S
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SMILES
O=S(C1=CC=CC([N+]([O-])=O)=C1)(NC2=C(C(NC3=CC(Cl)=C(Cl)C=C3)=O)C=C(Cl)C=C2)=O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
SOAT-IN-2 Related Classifications
HY-182767 Related Classifications
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