Sulfo DBCO-PEG3-NHS ester TEA
Sulfo DBCO-PEG3-NHS ester TEA is a click chemistry reagent containing an azide group. Sulfo DBCO-PEG3-NHS ester (TEA) is an analog of DBCO-Acid with PEG linker and a DBCO group. The DBCO group is commonly used for copper-free Click Chemistry reactions due to its strain promoted high energy. The hydrophilic PEG chain and sulfo group increase water solubility. The terminal carboxylic acid can react with primary amine groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research use only.
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- Formule: C41H55N5O13S
- Masse moléculaire:857.97
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Activité biologique
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Chemical Information
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Activité biologique
Description
Chemical Information
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Masse moléculaire 857.97
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Formule C41H55N5O13S
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SMILES
O=C(CCOCCOCCOCCC(NCC(S(=O)(O)=O)C(NCCC(N1C2=C(C#CC3=C(C1)C=CC=C3)C=CC=C2)=O)=O)=O)ON4C(CCC4=O)=O.CCN(CC)CC
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocole
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Pureté et documentation
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Sulfo DBCO-PEG3-NHS ester TEA Related Classifications
HY-148840A Related Classifications
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