1284240-77-2
Chemical Structure
Cy3.5
- CAS No.: 1284240-77-2
- Formula:C39H42N2O14S4
- Molecular Weight:891.02
IUPAC Name: 2-((1E,3E)-3-(3-(5-carboxypentyl)-1,1-dimethyl-6,8-disulfo-1,3-dihydro-2H-benzo[e]indol-2-ylidene)prop-1-en-1-yl)-3-ethyl-1,1-dimethyl-8-sulfo-1H-benzo[e]indol-3-ium-6-sulfonate
InChIKey: SMRJSACRIKPVSW-UHFFFAOYSA-N
SMILES: O=S(C1=CC(S(=O)([O-])=O)=C2C(C3=C([N+](CC)=C(/C=C/C=C4N(CCCCCC(O)=O)C5=C(C6=CC(S(=O)(O)=O)=CC(S(=O)(O)=O)=C6C=C5)C/4(C)C)C3(C)C)C=C2)=C1)(O)=O
Biological Activity: Cy3.5 is a cyanine-based red fluorescent dye (Ex/Em ≈ 560-581/600-612 nm). After covalent conjugation with proteins, Cy3.5 exhibits extraordinary fluorescence enhancement with minimal quenching even at high labeling ratios. Cy3.5 can form a FRET dye pair with Cy5.5, which maintains stable FRET efficiency in various biochemical buffers and deoxygenated systems. Cy3.5 is applicable to studies related to fluorescent labeling of antibodies/proteins, single-molecule FRET imaging of double-stranded DNA and nucleosomes, SPION functionalization, in vivo tracing, and other relevant research[1][2][3].
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Cy3.5 | Cy3.5 is a cyanine-based red fluorescent dye (Ex/Em ≈ 560-581/600-612 nm). After covalent conjugation with proteins, Cy3.5 exhibits extraordinary fluorescence enhancement with minimal quenching even at high labeling ratios. Cy3.5 can form a FRET dye pair with Cy5.5, which maintains stable FRET efficiency in various biochemical buffers and deoxygenated systems. Cy3.5 is applicable to studies related to fluorescent labeling of antibodies/proteins, single-molecule FRET imaging of double-stranded DNA and nucleosomes, SPION functionalization, in vivo tracing, and other relevant research. | |||||||||||||||||||||
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- [1]. Gruber HJ, et al. Anomalous fluorescence enhancement of Cy3 and cy3.5 versus anomalous fluorescence loss of Cy5 and Cy7 upon covalent linking to IgG and noncovalent binding to avidin. Bioconjugate chemistry. 2000;11(5):696-704. [Content Brief]
- [2]. Ghoneim M, et al. Single-Molecule Characterization of Cy3.5 -Cy5.5 Dye Pair for FRET Studies of Nucleic Acids and Nucleosomes. Journal of fluorescence. 2023 Mar;33(2):413-421. [Content Brief]
- [3]. Hellstern D, et al. Systemic distribution and elimination of plain and with Cy3.5 functionalized poly(vinyl alcohol) coated superparamagnetic maghemite nanoparticles after intraarticular injection in sheep in vivo. Journal of nanoscience and nanotechnology. 2006;6(9-10):3261-8.
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