148043-22-5
Chemical Structure
pH-Ap-1
- CAS 番号: 148043-22-5
- Formula:C20H20N2O2
- Molecular Weight:320.39
InChIKey: SDJKATWHQBUQEG-AATRIKPKSA-N
SMILES: O=C(OC1=C2)C(/C=C/C3=CC=NC=C3)=CC1=CC=C2N(CC)CC
Biological Activity: pH-Ap-1 is a fluorescent probe used for ratiometric monitoring of pH fluctuations during autophagy in living cells, and it can detect intracellular pH changes in the autophagy process. For fluorescence detection of pH-Ap-1, its excitation wavelength is 475 nm, with an emission wavelength of 538 nm under alkaline conditions and 645 nm under acidic conditions. For intracellular imaging, excitation is performed at 488 nm: the emission light ranging from 500-550 nm is collected via the green channel corresponding to 538 nm, while the emission light ranging from 625-750 nm is collected via the red channel corresponding to 645 nm. pH-Ap-1 exhibits excellent photostability and chemical stability[1].
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pH-Ap-1 | pH-Ap-1 is a fluorescent probe used for ratiometric monitoring of pH fluctuations during autophagy in living cells, and it can detect intracellular pH changes in the autophagy process. For fluorescence detection of pH-Ap-1, its excitation wavelength is 475 nm, with an emission wavelength of 538 nm under alkaline conditions and 645 nm under acidic conditions. For intracellular imaging, excitation is performed at 488 nm: the emission light ranging from 500-550 nm is collected via the green channel corresponding to 538 nm, while the emission light ranging from 625-750 nm is collected via the red channel corresponding to 645 nm. pH-Ap-1 exhibits excellent photostability and chemical stability. | |||||||||||||||||||||
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Keywords
