321862-17-3
Chemical Structure
5-TAMRA-NHS
- CAS No.: 321862-17-3
- Formula:C29H25N3O7
- Molecular Weight:527.52
InChIKey: CXYYHBMOVJJZTD-UHFFFAOYSA-N
SMILES: O=C(C1=CC=C2C(C(OC23C4=CC=C(N(C)C)C=C4OC5=CC(N(C)C)=CC=C53)=O)=C1)ON6C(CCC6=O)=O
Biological Activity: 5-TAMRA-NHS is a fluorescent dye composed of 5-TAMRA (HY-15942) and a hydroxysuccinimide (NHS) group. The NHS ester of 5-TAMRA-NHS forms amide bonds with primary amino groups, which is used to construct fluorescently labeled probes and conjugates. The labeled products are suitable for fluorescence anisotropy, confocal imaging and flow cytometry assays. 5-TAMRA-NHS can be used for fluorescent labeling and imaging (Ex/Em = 541/567 nm)[1][2].
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5-TAMRA-NHS | 5-TAMRA-NHS is a fluorescent dye composed of 5-TAMRA (HY-15942) and a hydroxysuccinimide (NHS) group. The NHS ester of 5-TAMRA-NHS forms amide bonds with primary amino groups, which is used to construct fluorescently labeled probes and conjugates. The labeled products are suitable for fluorescence anisotropy, confocal imaging and flow cytometry assays. 5-TAMRA-NHS can be used for fluorescent labeling and imaging (Ex/Em = 541/567 nm). | |||||||||||||||||||||
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References
- [1]. Shapiro AB, et al. 5-Carboxytetramethylrhodamine-Ampicillin Fluorescence Anisotropy-Based Assay of Escherichia coli Penicillin-Binding Protein 2 Transpeptidase Inhibition. ACS infectious diseases. 2019 Jun 14;5(6):863-872. [Content Brief]
- [2]. Rosier N, et al. Shedding Light on the D -Like Receptors: A Fluorescence-Based Toolbox for Visualization of the D and D Receptors. Chembiochem : a European journal of chemical biology. 2024 Jan 15;25(2):e202300658.
