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Art. -Nr.: HY-D3168

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PCL-2

PCL-2 is a reactive oxygen species (ROS)-responsive fluorescent probe. PCL-2 reacts with ROS to release 6-hydroxy-2-cyanobenzothiazole (HCBT), which then reacts with D-cysteine to form firefly luciferin in situ. PCL-2 must be used in conjunction with IETDC (HY-D3169) to detect the co-existence of hydrogen peroxide and caspase 8. PCL-2 is applicable to studies related to acute inflammation.

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CAS. Nr.: 1421277-81-7
Formel: C₁₅H₁₁BN₂O₃S
Molecular Weight:310.14

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

PCL-2 Chemical Structure

CAS. Nr.: 1421277-81-7

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
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- In Vitro
- In Vivo
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Beschreibung

In Vitro

PCL-2 (5 μM; 5, 20, 40, or 60 min) selectively produces a ca. 50-fold increase in bioluminescent signal in response to H₂O₂, but not other biologically relevant ROS, in a cell-free system^[1].

PCL-2 (5 μM; 60 min) produces a linearly increasing bioluminescent signal in response to 1-100 μM H₂O₂ in a cell-free system^[1].

PCL-2 (25 μM; 2 h) produces a linearly increasing, statistically significant bioluminescent signal in response to 0-100 μM H₂O₂ in PC3M-luc cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Mice

PCL-2 (0.05 μmol; i.p.) produces a dose-dependent, 10-fold maximum bioluminescent turn-on signal in response to exogenous hydrogen peroxide in healthy FVB-luc⁺ mice, which is attenuated by a hydrogen peroxide scavenger^[1].

Mice

PCL-2 (0.05 μmol; i.p.) detects a ~3.7-fold increase in endogenous hydrogen peroxide (H₂O₂) levels during lipopolysaccharide-induced acute inflammation in female FVB-luc+ mice^[1].

Mice

PCL-2 (0.05 μmol; i.p.), in combination with IETDC, detects a ~2.7-fold increase in simultaneous endogenous hydrogen peroxide and caspase 8 activity during LPS (HY-D1056)-induced acute inflammation in female FVB-luc+ mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

FVB-luc+ (FVB-Tg(CAG-luc,-GFP)L2G85Chco/J) (female, 2-5 months)^[1]
Dosage:

0.05 μmol (co-administered with 0.05 μmol D-cysteine)
Administration:

intraperitoneal injection
Result:

Produced a ~3.7-fold increase in total photon flux in lipopolysaccharide-challenged mice compared to saline-treated control mice.
Reduced this signal by ~55% when co-administered with apocynin relative to signal from lipopolysaccharide alone.

Animal Model:

FVB-luc+ (FVB-Tg(CAG-luc,-GFP)L2G85Chco/J) (female, 2-5 months)^[1]
Dosage:

0.05 μmol (co-administered with 0.05 μmol IETDC)
Administration:

intraperitoneal injection
Result:

Produced a ~2.7-fold increase in total photon flux in lipopolysaccharide-challenged mice compared to saline-treated control mice.
Reduced this signal by ~30% when co-administered with ascorbic acid and z-VD(OMe)-OPh relative to signal from lipopolysaccharide alone.

Related Protocols :

Chemical Information

CAS. Nr. 1421277-81-7
Molecular Weight 310.14
Formel C₁₅H₁₁BN₂O₃S
SMILES

N#CC1=NC2=CC=C(C=C2S1)OCC3=CC=C(C=C3)B(O)O
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Reinheit & Dokumentation

Handling Instructions (2659 KB)

Verweise

[1]. Van de Bittner GC, et al. Strategy for dual-analyte luciferin imaging: in vivo bioluminescence detection of hydrogen peroxide and caspase activity in a murine model of acute inflammation. J Am Chem Soc. 2013;135(5):1783-1795. [Content Brief]

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Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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PCL-2

Biologische Aktivität

Beschreibung

In Vitro

In Vivo

Chemical Information

Reinheit & Dokumentation

Verweise

Calculators

Help & FAQs

PCL-2 Related Classifications

HY-D3168 Related Classifications

Bioz

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