Art. -Nr.: HY-151576

PRMT5:MEP50 PPI

Name: PRMT5:MEP50 PPI
Brand: MedChemExpress
SKU: HY-151576
Price: 650.00 USD
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PRMT5:MEP50 PPI is a novel PRMT5:MEP50 protein-protein interaction (PRMT5:MEP50 PPI) inhibitor, shows anti-tumor activity and anti-proliferative activity of lung and prostate cancer cells.

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Reinheit: 98.09%
CAS. Nr.: 3031536-71-4
Formel: C₂₄H₂₂N₄O₄
Molecular Weight:430.46

Speicherung:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

PRMT5:MEP50 PPI Chemical Structure

CAS. Nr.: 3031536-71-4

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Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

PRMT5:MEP50 PPI is a novel PRMT5:MEP50 protein-protein interaction (PRMT5:MEP50 PPI) inhibitor, shows anti-tumor activity and anti-proliferative activity of lung and prostate cancer cells^[1].

Alle Histone Methyltransferase Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	447 nM Compound: 17	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	[PMID: 36206451]
LNCaP	IC₅₀	430 nM Compound: 17	Induction of cell death in human LNCaP cells incubated for 72 hrs by MTT assay Induction of cell death in human LNCaP cells incubated for 72 hrs by MTT assay	[PMID: 36206451]

In Vitro

PRMT5:MEP50 PPI (0-10 μM; 72 h) inhibits the growth of prostate cancer cells^[1].
PRMT5:MEP50 PPI (250-1000 nM; 72 h) decreases the expression of global arginine 3 residue of histone H4 (H4R3me2s) level in prostate cancer cells^[1].
PRMT5:MEP50 PPI (500 nM; 72 h) de-represseses the PRMT5: MEP50-regulated IVL gene of prostate cancer cells^[1].
PRMT5:MEP50 PPI treatment inhibits multiple PRMT5-regulated pathways critical to the survival and proliferation of lung and prostate cancer cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

LNCaP cells
Concentration:

0-10 μM
Incubation Time:

72 h
Result:

Showed IC₅₀ value of 430.2 nM.

Western Blot Analysis^[1]

Cell Line:

LNCaP cells
Concentration:

250, 500 and 1000 nM
Incubation Time:

72 h
Result:

Observed global arginine 3 residue of histone H4 (H4R3me2s) decreased by 65%.

RT-PCR^[1]

Cell Line:

LNCaP cells
Concentration:

500 nM
Incubation Time:

72 h
Result:

De-repressesed the PRMT5: MEP50-regulated IVL gene without significantly altering the expression of the PRMT5: pICln-regulated AR gene.

Chemical Information

CAS. Nr. 3031536-71-4
Appearance Solid
Molecular Weight 430.46
Formel C₂₄H₂₂N₄O₄
Color White to off-white
SMILES

CCC1=NOC(C)=C1COC2=CC(C(NNC(C3=NC4=C(C=C3)C=CC=C4)=O)=O)=CC=C2
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 200 mg/mL (464.62 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3231 mL

11.6155 mL

23.2310 mL

5 mM

0.4646 mL

2.3231 mL

4.6462 mL

10 mM

0.2323 mL

1.1615 mL

2.3231 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 5 mg/mL (11.62 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (271 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Asberry AM, et al. Discovery and Biological Characterization of PRMT5:MEP50 Protein-Protein Interaction Inhibitors. J Med Chem. 2022 Oct 7. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.3231 mL	11.6155 mL	23.2310 mL	58.0774 mL
	5 mM	0.4646 mL	2.3231 mL	4.6462 mL	11.6155 mL
	10 mM	0.2323 mL	1.1615 mL	2.3231 mL	5.8077 mL
	15 mM	0.1549 mL	0.7744 mL	1.5487 mL	3.8718 mL
	20 mM	0.1162 mL	0.5808 mL	1.1615 mL	2.9039 mL
	25 mM	0.0929 mL	0.4646 mL	0.9292 mL	2.3231 mL
	30 mM	0.0774 mL	0.3872 mL	0.7744 mL	1.9359 mL
	40 mM	0.0581 mL	0.2904 mL	0.5808 mL	1.4519 mL
	50 mM	0.0465 mL	0.2323 mL	0.4646 mL	1.1615 mL
	60 mM	0.0387 mL	0.1936 mL	0.3872 mL	0.9680 mL
	80 mM	0.0290 mL	0.1452 mL	0.2904 mL	0.7260 mL
	100 mM	0.0232 mL	0.1162 mL	0.2323 mL	0.5808 mL

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PRMT5:MEP50 PPI

Alle Histone Methyltransferase Isoform-spezifische Produkte anzeigen