UT-B-IN-1
Based on 1 publication(s) in Google Scholar
UT-B-IN-1 (UTBINH-14) is a reversible, competitive and selective urea transporter-B (UT-B) inhibitor with IC50 values of 10 and 25 nM for human and mouse UT-B, respectively. UT-B-IN-1 shows low toxicity and high selectivity for UT-B over UT-A isoforms. UT-B-IN-1 increases urine output and reduces urine osmolality of mice. UT-B-IN-1 can be used for diuretic mechanism research.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit: 98.07%
- CAS. Nr.: 892742-76-6
- Formel: C20H17N5O2S3
- Molecular Weight:455.58
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) UT-B-IN-1
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Biologische Aktivität
IC50: 10 nM (human UT-B); 25 nM (mouse UT-B)[1]
UT-B-IN-1 (1-1000 nM) inhibits urea efflux in erythrocytes preloaded with urea with an IC50 value of 26.7 nM[1]. UT-B-IN-1 inhibits water transport in AQP1-null erythrocytes[1]. UT-B-IN-1 (0-10 μM;24 h) shows no cytotoxicity to MDCK cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Madin-Darby canine kidney (MDCK) cell line
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Concentration:0-10 μM
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Incubation Time:24 hours
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Result:Exhibited no cytotoxic effect to MDCK cells.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male mice (wild-type or UT-B knockout)[1]
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Dosage:300 μg
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Administration:Intraperitoneal injection, 300 μg, once
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Result:Reduced urine osmolality and urea concentration in wild-type mice with V2-receptor agonist dDAVP injection. Increased urine volume and reduced urine osmolality and urea concentration in mice with free access to food and water but without dDAVP.
Chemical Information
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CAS. Nr. 892742-76-6
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Appearance Solid
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Molecular Weight 455.58
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Formel C20H17N5O2S3
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Color White to off-white
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SMILES
O=S(C1=C2N=C(NCC3=CC=CS3)C4=C(C=CS4)N2N=N1)(C5=CC=C(CC)C=C5)=O
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
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Metabolites
Modulation of Urea Transport Attenuates TLR2-Mediated Microglial Activation and Upregulates Microglial Metabolism In Vitro. [Abstract]2024 Nov 17;14(11):634. PMID: 39590870
Lösungsmittel & Löslichkeit
DMSO : 100 mg/mL (219.50 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (5.49 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
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Data Sheet (278 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1950 mL | 10.9750 mL | 21.9500 mL | 54.8751 mL |
| 5 mM | 0.4390 mL | 2.1950 mL | 4.3900 mL | 10.9750 mL | |
| 10 mM | 0.2195 mL | 1.0975 mL | 2.1950 mL | 5.4875 mL | |
| 15 mM | 0.1463 mL | 0.7317 mL | 1.4633 mL | 3.6583 mL | |
| 20 mM | 0.1098 mL | 0.5488 mL | 1.0975 mL | 2.7438 mL | |
| 25 mM | 0.0878 mL | 0.4390 mL | 0.8780 mL | 2.1950 mL | |
| 30 mM | 0.0732 mL | 0.3658 mL | 0.7317 mL | 1.8292 mL | |
| 40 mM | 0.0549 mL | 0.2744 mL | 0.5488 mL | 1.3719 mL | |
| 50 mM | 0.0439 mL | 0.2195 mL | 0.4390 mL | 1.0975 mL | |
| 60 mM | 0.0366 mL | 0.1829 mL | 0.3658 mL | 0.9146 mL | |
| 80 mM | 0.0274 mL | 0.1372 mL | 0.2744 mL | 0.6859 mL | |
| 100 mM | 0.0220 mL | 0.1098 mL | 0.2195 mL | 0.5488 mL |
UT-B-IN-1 Related Classifications
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