MQAE
Based on 18 publication(s) in Google Scholar
MQAE is a chloride ion (Cl-) fluorescent probe that can be used to measure chloride concentrations. The fluorescence intensity of MQAE decreases proportionally as Cl- ions increase. MQAE has high cell permeability and is suitable for fluorescence detection such as confocal microscopy and flow cytometry (Ex/Em=350/460 nm).
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- Pureté: 99.42%
- CAS No.: 162558-52-3
- Formule: C14H16BrNO3
- Masse moléculaire:326.19
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Stockage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light)
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) MQAE
More- Nat Neurosci. 2023 May;26(5):751-764. [Abstract]
- CCS Chem. 20 Jul 2021.
- Int J Biol Macromol. 2025 Mar 27:142512. [Abstract]
- J Transl Med. 2024 Nov 16;22(1):1034. [Abstract]
- Diabetes Res Clin Pract. 2022 Aug:190:109970. [Abstract]
- J Med Chem. 2025 Aug 28;68(16):17705-17722. [Abstract]
- J Med Chem. 2025 Aug 28;68(16):17971-17989. [Abstract]
- Biochem Pharmacol. 2025 Feb 12:234:116801. [Abstract]
- Biochem Pharmacol. 2022 Dec:206:115326. [Abstract]
- Cells. 2025 Oct 15;14(20):1601. [Abstract]
- Cells. 2020 Apr 22;9(4):1045. [Abstract]
- Commun Biol. 2025 Jan 14;8(1):51. [Abstract]
- Front Nutr. 2021 Mar 29;8:638390. [Abstract]
- J Virol. 2026 Mar 24;100(3):e0220025. [Abstract]
- Heliyon. 2024 Jun 13;10(12):e32481. [Abstract]
- Toxicol Appl Pharmacol. 2025 May 11:117383. [Abstract]
- bioRxiv. 2025 Jul 18.
- Oxid Med Cell Longev. 2022 Apr 19;2022:4608914. [Abstract]
Customer Validation & Images
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Cell Imaging/Staining
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
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IF
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Cell Imaging/Staining
Activité biologique
Guide (The following is our recommended experimental protocol. This protocol is for reference only and should be modified according to your specific needs).
1 Preparation of MQAE working solution
1.1 Preparation of the stock solution
Dissolve 1 mg MQAE in 0.3066 mL DMSO to obtain 10 mM of MQAE.
Note: It is recommended to store the stock solution at -20°C -80°C away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of MQAE working solution
Dilute the stock solution in Krebs-hepes buffer (20 mM HEPES, 128 mM NaCl, 2.5 mM KCl, 2.7 mM CaCl2, 1 mM MgCl2, 16 mM glucose, pH 7.4) to obtain 5-10 mM of MQAE working solution.
Note: Please adjust the concentration of MQAE working solution according to the actual situation.
2 Cell staining
2.1 For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of MQAE working solution, and then incubate at room temperature for 30 minutes.
2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with serum-free cell culture medium or PBS.
3 Storage
-20°C, 1 year. Protect from light.
4 Precautions
4.1 It is recommended to store the stock solution at -20°C or -80°Caway from light and avoid repetitive freeze-thaw cycles.
4.2 Please adjust the concentration of MQAE working solution according to the actual situation.
4.3 This product is for R&D use only, not for drug, household, or other uses.
4.4 For your safety and health, please wear a lab coat and disposable gloves to operate.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Chemical Information
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CAS No. 162558-52-3
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Appearance Solid
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Masse moléculaire 326.19
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Formule C14H16BrNO3
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Color Light yellow to khaki
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SMILES
COC1=CC2=CC=C[N+](CC(OCC)=O)=C2C=C1.[Br-]
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light)
Publications (18)
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Journal Impact Factor
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Most Recent
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Nat Neurosci
Emergence of consciousness from anesthesia through ubiquitin degradation of KCC2 in the ventral posteromedial nucleus of the thalamus. [Abstract]2023 May;26(5):751-764. PMID: 36973513 -
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Int J Biol Macromol
Polysaccharide from Atractylodes macrocephala Koidz. alleviates pyrotinib-induced diarrhea through regulating cAMP/LKB1/AMPK/CFTR pathway and restoring gut microbiota and metabolites. [Abstract]2025 Mar 27:142512. PMID: 40157659 -
J Transl Med
LRRC8A drives NADPH oxidase-mediated mitochondrial dysfunction and inflammation in allergic rhinitis. [Abstract]2024 Nov 16;22(1):1034. PMID: 39550567
MQAE purchased from MedChemExpress. Usage Cited in: J Transl Med. 2024 Nov 16;22(1):1034. [Abstract]
Measurement of Cl− expression in HNEPCs using MQAE (5 mM, 1 h) (scale bar = 50 μm).
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Diabetes Res Clin Pract
2022 Aug:190:109970. PMID: 35792204 -
J Med Chem
Discovery of Tamsulosin Derivatives with Shifted Selectivity from the α1-Adrenergic Receptor to ANO1 as Potent Antiosteoporotic Agents. [Abstract]2025 Aug 28;68(16):17705-17722. PMID: 40794435 -
J Med Chem
2025 Aug 28;68(16):17971-17989. PMID: 40788257 -
Biochem Pharmacol
Tamibarotene directly targets the NACHT domain of NLRP3 to alleviate acute myocardial infarction. [Abstract]2025 Feb 12:234:116801. PMID: 39952330 -
Biochem Pharmacol
Bortezomib inhibits NLRP3 inflammasome activation and NF-κB pathway to reduce psoriatic inflammation. [Abstract]2022 Dec:206:115326. PMID: 36326534 -
Cells
Mitochondrial Fragmentation Induced by the CFTR Modulators Lumacaftor and Ivacaftor in Immortalized Cystic Fibrosis Cell Lines. [Abstract]2025 Oct 15;14(20):1601. PMID: 41148816
MQAE purchased from MedChemExpress. Usage Cited in: Cells. 2025 Oct 15;14(20):1601. [Abstract]
Stern–Volmer constant for MQAE (5 mM, 1 h).
MQAE purchased from MedChemExpress. Usage Cited in: Cells. 2025 Oct 15;14(20):1601. [Abstract]
Bars and dots (replicates) showed the quantification of MQAE (5 mM) fluorescence normalized to control in C38 cells.
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Cells
Association Analysis of Single-Cell RNA Sequencing and Proteomics Reveals a Vital Role of Ca2+ Signaling in the Determination of Skeletal Muscle Development Potential. [Abstract]2020 Apr 22;9(4):1045. PMID: 32331484
MQAE purchased from MedChemExpress. Usage Cited in: Cells. 2020 Apr 22;9(4):1045. [Abstract]
The concentration of cytoplasmic Cl− represented by the fluorescence of N-(ethoxycarbonylmethyl)-6-methoxyquinolinium bromide (MQAE, 5 Mm, 45 min).
MQAE purchased from MedChemExpress. Usage Cited in: Cells. 2020 Apr 22;9(4):1045. [Abstract]
The concentration of cytoplasmic Cl− represented by the fluorescence of N-(ethoxycarbonylmethyl)-6-methoxyquinolinium bromide (MQAE, 5 Mm, 45 min).
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Commun Biol
Chloride intracellular channel CLIC3 mediates fibroblast cellular senescence by interacting with ERK7. [Abstract]2025 Jan 14;8(1):51. PMID: 39809890 -
Front Nutr
Colloidal Particles in Tuna Head Soup: Chemical Localization, Structural Change, and Antioxidant Property. [Abstract]2021 Mar 29;8:638390. PMID: 33855041
MQAE purchased from MedChemExpress. Usage Cited in: Front Nutr. 2021 Mar 29;8:638390. [Abstract]
Nile Red, MQAE, and CoroNa™ Green are used to stain the triglycerides (TG), chloride ions (Cl-), and sodium ions (Na+), respectively.
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J Virol
DIDS modulates VDAC1 oligomerization to suppress intrinsic apoptosis and attenuates in vitro and in vivo RSV infection. [Abstract]2026 Mar 24;100(3):e0220025. PMID: 41670372 -
Heliyon
Sevoflurane-induced regulation of NKCC1/KCC2 phosphorylation through activation of Spak/OSR1 kinase and cognitive impairment in ischemia-reperfusion injury in rats. [Abstract]2024 Jun 13;10(12):e32481. PMID: 38975218 -
Toxicol Appl Pharmacol
Harnessing FDA-approved dipyridamole to inhibit NLRP3 inflammasome and improve outcomes of acute lung injury in sepsis. [Abstract]2025 May 11:117383. PMID: 40360054 -
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Oxid Med Cell Longev
Attenuation of ROS/Chloride Efflux-Mediated NLRP3 Inflammasome Activation Contributes to Alleviation of Diabetic Cardiomyopathy in Rats after Sleeve Gastrectomy. [Abstract]2022 Apr 19;2022:4608914. PMID: 35498125
Solvant et solubilité
H2O : 100 mg/mL (306.57 mM; Need ultrasonic)
DMSO : ≥ 35 mg/mL (107.30 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureté et documentation
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Fiche technique (276 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Portuguese - PT (394 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Kovalchuk Y, et al. Two-photon chloride imaging using MQAE in vitro and in vivo. Cold Spring Harb Protoc. 2012 Jul 1;2012(7):778-85. [Content Brief]
[2]. Andersson C, et al. Determination of chloride efflux by X-ray microanalysis versus MQAE-fluorescence. Microsc Res Tech. 2 [Content Brief]
[3]. Koncz C, et al. Use of MQAE for measurement of intracellular [Cl-] in cultured aortic smooth muscle cells. Am J Physiol. 1994 Dec;267(6 Pt 2):H2114-23. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO / H2O | 1 mM | 3.0657 mL | 15.3285 mL | 30.6570 mL | 76.6424 mL |
| 5 mM | 0.6131 mL | 3.0657 mL | 6.1314 mL | 15.3285 mL | |
| 10 mM | 0.3066 mL | 1.5328 mL | 3.0657 mL | 7.6642 mL | |
| 15 mM | 0.2044 mL | 1.0219 mL | 2.0438 mL | 5.1095 mL | |
| 20 mM | 0.1533 mL | 0.7664 mL | 1.5328 mL | 3.8321 mL | |
| 25 mM | 0.1226 mL | 0.6131 mL | 1.2263 mL | 3.0657 mL | |
| 30 mM | 0.1022 mL | 0.5109 mL | 1.0219 mL | 2.5547 mL | |
| 40 mM | 0.0766 mL | 0.3832 mL | 0.7664 mL | 1.9161 mL | |
| 50 mM | 0.0613 mL | 0.3066 mL | 0.6131 mL | 1.5328 mL | |
| 60 mM | 0.0511 mL | 0.2555 mL | 0.5109 mL | 1.2774 mL | |
| 80 mM | 0.0383 mL | 0.1916 mL | 0.3832 mL | 0.9580 mL | |
| 100 mM | 0.0307 mL | 0.1533 mL | 0.3066 mL | 0.7664 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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