Cholesterol 24-hydroxylase-IN-3

Name: Cholesterol 24-hydroxylase-IN-3
Brand: MedChemExpress
SKU: HY-174332
Rating: 4.5 (1 reviews)

Cat. No.: HY-174332 Purity: 98.74%: Data Sheet
COA

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Handling Instructions
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Cholesterol 24-hydroxylase-IN-3 is an orally active, selective, and blood-brain barrier-penetrant CH24H inhibitor (IC₅₀ = 23 nM) belonging to 1,3-oxazole derivatives. Cholesterol 24-hydroxylase-IN-3 competitively inhibits CH24H enzyme activity by using the 1,3-oxazole nitrogen atom to coordinate the heme iron and the cyclopropyl group occupying the hydrophobic pocket. Cholesterol 24-hydroxylase-IN-3 can be used for research on epilepsy and other neurological diseases.

For research use only. We do not sell to patients.

Cholesterol 24-hydroxylase-IN-3 Chemical Structure

CAS No. : 1630746-27-8

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Cytochrome P450 Isoform Specific Products:

View All Isoforms

CYP1 CYP2 CYP3 CYP4 CYP11 CYP17 Aromatase/CYP19A1 CYP26 CYP51 CYP1A2 CYP2D6 CYP2C9 CYP2C19 CYP3A CYP3A4 CYP17A1

Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Cholesterol 24-hydroxylase-IN-3 (Compound 4L) (10 μM, 1 h) is selective for CH24H, exhibits low inhibitory rates against other CYP enzymes, with inhibitory rates of 7.3 %, 10 %, 8.5 %, and 49 % against CYP3A4, CYP2C8, CYP2C9, and CYP2D6, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Cholesterol 24-hydroxylase-IN-3 (Compound 4L) (30 mg/kg, i.g, single dose) can effectively penetrate the blood-brain barrier and reduce the level of 24S-hydroxycholesterol (24HC) in the brain in mice, providing a new treatment strategy for neurological diseases^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6N nude female mice (7 weeks)^[1]
Dosage:	30 mg/kg
Administration:	Oral gavage (i.g.), samples were collected at 1, 8, and 24 h after administration
Result:	Had excellent blood-brain barrier penetration ability (brain/plasma ratio = 1.0), and concentrations in the brain and plasma were 0.902 µM and 0.868 µM after 8 hours administration. Significantly inhibited the CH24H enzyme function in the brain and reduced the level of cholesterol metabolite 24HC; compared to the control group, it significantly suppressed brain 24HC levels by 21%.

Molecular Weight

297.23

Formula

C₁₃H₁₀F₃N₃O₂

CAS No.

1630746-27-8

Appearance

Solid

Color

White to off-white

SMILES

O=C(C1CC1)NC2=CC(C(F)(F)F)=CC(C3=CN=CO3)=N2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (84.11 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.3644 mL	16.8220 mL	33.6440 mL
5 mM	0.6729 mL	3.3644 mL	6.7288 mL
10 mM	0.3364 mL	1.6822 mL	3.3644 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.74%

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Data Sheet (276 KB) SDS (251 KB)

COA (253 KB) HNMR (582 KB) RP-HPLC (258 KB) MS (69 KB)

Handling Instructions (2659 KB)

References

[1]. Zheng Yang, et al. Design, synthesis, and in vitro and in vivo anti-drug resistant cervical cancer activity of novel licochalcone A derivatives based on dual targeting of VEGFR-2/P-gp. Eur J Med Chem. 2025 May 28 163:108639. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.3644 mL	16.8220 mL	33.6440 mL	84.1099 mL
	5 mM	0.6729 mL	3.3644 mL	6.7288 mL	16.8220 mL
	10 mM	0.3364 mL	1.6822 mL	3.3644 mL	8.4110 mL
	15 mM	0.2243 mL	1.1215 mL	2.2429 mL	5.6073 mL
	20 mM	0.1682 mL	0.8411 mL	1.6822 mL	4.2055 mL
	25 mM	0.1346 mL	0.6729 mL	1.3458 mL	3.3644 mL
	30 mM	0.1121 mL	0.5607 mL	1.1215 mL	2.8037 mL
	40 mM	0.0841 mL	0.4205 mL	0.8411 mL	2.1027 mL
	50 mM	0.0673 mL	0.3364 mL	0.6729 mL	1.6822 mL
	60 mM	0.0561 mL	0.2804 mL	0.5607 mL	1.4018 mL
	80 mM	0.0421 mL	0.2103 mL	0.4205 mL	1.0514 mL