Coumarin 343 azide
Coumarin 343 azide is a fluorescent dye used for the fluorescent labeling of alkyne-containing biomolecules based on click chemistry. Its detection mechanism relies on the copper (I)-catalyzed azide-alkyne cycloaddition (CuAAC), a type of click reaction: under mild aqueous conditions, the azide group of this dye selectively reacts with the alkyne moiety of propargyl geldanamycin to form a triazole bond that conjugates the fluorescent dye to the target biomolecule; fluorescence is generated upon successful conjugation to the target.
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- CAS No.: 1807503-82-7
- Formula: C22H27N5O5
- Molecular Weight:441.48
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Coumarin 343 azide is widely used in click chemistry to label biomolecules bearing alkyne tags, and can be paired with fluorescein (FAM) as a highly efficient fluorescence resonance energy transfer donor.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 1807503-82-7
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Molecular Weight 441.48
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Formula C22H27N5O5
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SMILES
[N-]=[N+]=NCCOCCOCCNC(C1=CC2=C(OC1=O)C(CCCN3CCC4)=C3C4=C2)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Coumarin 343 azide Related Classifications
HY-D3521 Related Classifications
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