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Cat. No.: HY-D2200

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Cy5.5(Me)-C3-DBCO

Cy5.5(Me)-C3-DBCO is a click chemistry reagent containing an cycloalkynes group. DBCO group enables copper free biocompatible click chemistry with fast reaction kinetics and good stability.

For research use only. We do not sell to patients.

Formula: C₅₈H₅₇ClN₄O₂
Molecular Weight:877.55

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (270 KB)

Cy5.5(Me)-C3-DBCO Chemical Structure

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity

Description

Cy5.5(Me)-C3-DBCO is a click chemistry reagent containing an cycloalkynes group. DBCO group enables copper free biocompatible click chemistry with fast reaction kinetics and good stability^[1].

Chemical Information

Molecular Weight 877.55
Formula C₅₈H₅₇ClN₄O₂
SMILES

CC1(C2=C(C=CC3=CC=CC=C32)[N+](CCCCCC(NCCC(N4C5=CC=CC=C5C#CC6=CC=CC=C6C4)=O)=O)=C1/C=C/C=C/C=C7C(C)(C8=C(C=CC9=CC=CC=C98)N\7C)C)C.[Cl-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Protocols

All Cell Biology

EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (270 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Sangmin Lee, et al. In vivo stem cell tracking with imageable nanoparticles that bind bioorthogonal chemical receptors on the stem cell surface. Biomaterials. 2017 Sep:139:12-29. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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