1. Dye Reagents
  2. Cyanine 3.5 chloride

Cyanine 3.5 chloride (Synonyms: Cy3.5 NHS ester chloride; Cy 3.5 chloride)

Cat. No.: HY-D1596
Handling Instructions

Cyanine 3.5 (Cy3.5 NHS ester) chloride is an analog of Cy3.5 fluorophore. Cyanine 3.5 chloride is a reactive, red fluorescent dye. Cyanine 3.5 chloride is used for labeling of amino-groups in peptides, proteins, and oligonucleotides. (λex=591 nm, λem=604 nm).

For research use only. We do not sell to patients.

Cyanine 3.5 chloride Chemical Structure

Cyanine 3.5 chloride Chemical Structure

CAS No. : 2231670-86-1

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Description

Cyanine 3.5 (Cy3.5 NHS ester) chloride is an analog of Cy3.5 fluorophore. Cyanine 3.5 chloride is a reactive, red fluorescent dye. Cyanine 3.5 chloride is used for labeling of amino-groups in peptides, proteins, and oligonucleotides. (λex=591 nm, λem=604 nm)[1][2].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Labeling of Cells: 1. Culture HepG2 cells in DMEM/HIGH GLUCOSE medium (containing 10% FBS, 100 U/mL penicillin G and 100 μg/mL streptomycin sulfate) at 37oC in 5% CO2 atmosphere. 2. Add 1×106 HepG2 cells with logarithmic half-life in a confocal culture dish. Incubate cells at 37oC, 5% CO2, for 36 h. 3. Add 200 μL of 10 μg/mL Hoechst fluorescent dye 33342. Incubate cells at 37o, 5% CO2, for 20 minutes. 4. Changed the medium with 200 μL of Cy3.5-loaded FA-PEG-RH nanomicelles. 5. Take cell photos with a fluorescence microscope under 350 nm and 550 nm fluorescence excitation after 1h, 4 h, 8 h, and 16 h, respectively.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

690.27

Formula

C42H44ClN3O4

CAS No.
SMILES

O=C(CCCCC[N+]1=C(/C=C/C=C2N(C)C3=C(C/2(C)C)C4=C(C=CC=C4)C=C3)C(C)(C)C5=C1C=CC6=C5C=CC=C6)ON7C(CCC7=O)=O.[Cl-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation
References
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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× = ×
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Product Name:
Cyanine 3.5 chloride
Cat. No.:
HY-D1596
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