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Cyanine3 maleimide tetrafluoroborate 

Cat. No.: HY-D1618
Handling Instructions

Cyanine3 maleimide tetrafluoroborate is a specific labeling reagent for sulfhydryl groups with the λex of 550 nm and λem of 580 nm. Cyanine3 maleimide tetrafluoroborate can be used for protein and peptide labeling.

For research use only. We do not sell to patients.

Cyanine3 maleimide tetrafluoroborate Chemical Structure

Cyanine3 maleimide tetrafluoroborate Chemical Structure

CAS No. : 2755154-93-7

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Description

Cyanine3 maleimide tetrafluoroborate is a specific labeling reagent for sulfhydryl groups with the λex of 550 nm and λem of 580 nm. Cyanine3 maleimide tetrafluoroborate can be used for protein and peptide labeling[1][2].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Labeling of Protein: 1.The protein concentration was adjusted to 1 mg/mL for 100 μL. 2. A 100 μL aliquot of the protein solution was mixed with Cyanine3 maleimide tetrafluoroborate (8 μg) in DMSO (2 μL). 3. Incubated overnight at 4 °C in the dark. 4. Labeled proteins were desalted using a G25 desalting column to remove excessive Cyanine3 maleimide tetrafluoroborate. 5. Fluorescence intensities (FI) of labeled proteins were measured at excitation and emission wavelengths of 550 nm and 570 nm, respectively. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

666.56

Formula

C36H43BF4N4O32-

CAS No.
SMILES

CN1C2=CC=CC=C2C(C)(/C1=C\C=C\C3=[N-](C4=CC=CC=C4C3(C)C)CCCCCC(NCCN5C(C=CC5=O)=O)=O)C.[F-][B+3]([F-])([F-])[F-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

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Product Name:
Cyanine3 maleimide tetrafluoroborate
Cat. No.:
HY-D1618
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