Cyanine3 maleimide tetrafluoroborate 

Cyanine3 maleimide tetrafluoroborate is a specific labeling reagent for sulfhydryl groups with the λ_ex of 550 nm and λ_em of 580 nm. Cyanine3 maleimide tetrafluoroborate can be used for protein and peptide labeling^[1][2].

Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Labeling of Protein:
1.The protein concentration was adjusted to 1 mg/mL for 100 μL.
2. A 100 μL aliquot of the protein solution was mixed with Cyanine3 maleimide tetrafluoroborate (8 μg) in DMSO.
3. Incubated overnight at 4°C in the dark.
4. Labeled proteins were desalted using a G25 desalting column to remove excessive Cyanine3 maleimide tetrafluoroborate.
5. Fluorescence intensities (FI) of labeled proteins were measured at excitation and emission wavelengths of 550 nm and 570 nm, respectively.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

666.56

C₃₆H₄₃BF₄N₄O₃

2755154-93-7

570

550

O=C1C=CC(N1CCNC(CCCCC[N+]2=C(/C=C/C=C3N(C)C4=C(C\3(C)C)C=CC=C4)C(C)(C)C5=C2C=CC=C5)=O)=O.[F-][B+3]([F-])([F-])[F-]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Zartner L, et al. Membrane protein channels equipped with a cleavable linker for inducing catalysis inside nanocompartments. J Mater Chem B. 2021 Nov 10;9(43):9012-9022.  [Content Brief]

  [2]. He J, et al. The effect of meat processing methods on changes in disulfide bonding and alteration of protein structures: impact on protein digestion products. RSC Adv. 2018 May 15;8(31):17595-17605.  [Content Brief]