DBCO-CONH-S-S-NHS ester
Based on 1 Customer Validation
DBCO-CONH-S-S-NHS ester is a cleavable ADC linker used in the synthesis of antibody-drug conjugates (ADCs). DBCO-CONH-S-S-NHS ester is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.
For research use only. We do not sell to patients.
- Purity : 99.18%
- CAS No.: 1435934-53-4
- Formula: C28H27N3O6S2
- Molecular Weight:565.66
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Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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DBCO-PEG3-aminePROTAC Linker -
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DBCO-PEG3-NHSPROTAC Linker -
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DBCO-PEG2-NHS esterAzide Compound -
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DBCO-PEG1-NHS esterPROTAC Linkers
Biological Activity
Description
IC50 & Target
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Disulfide Cleavable Linker |
Cleavable Linker |
In Vitro
ADCs are comprised of an antibody to which is attached an ADC cytotoxin through an ADC linker[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 1435934-53-4
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Appearance Solid
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Molecular Weight 565.66
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Formula C28H27N3O6S2
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Color White to light yellow
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SMILES
O=C(N1C2=CC=CC=C2C#CC3=CC=CC=C3C1)CCC(NCCSSCCC(ON4C(CCC4=O)=O)=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (176.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
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Data Sheet (270 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7678 mL | 8.8392 mL | 17.6785 mL | 44.1962 mL |
| 5 mM | 0.3536 mL | 1.7678 mL | 3.5357 mL | 8.8392 mL | |
| 10 mM | 0.1768 mL | 0.8839 mL | 1.7678 mL | 4.4196 mL | |
| 15 mM | 0.1179 mL | 0.5893 mL | 1.1786 mL | 2.9464 mL | |
| 20 mM | 0.0884 mL | 0.4420 mL | 0.8839 mL | 2.2098 mL | |
| 25 mM | 0.0707 mL | 0.3536 mL | 0.7071 mL | 1.7678 mL | |
| 30 mM | 0.0589 mL | 0.2946 mL | 0.5893 mL | 1.4732 mL | |
| 40 mM | 0.0442 mL | 0.2210 mL | 0.4420 mL | 1.1049 mL | |
| 50 mM | 0.0354 mL | 0.1768 mL | 0.3536 mL | 0.8839 mL | |
| 60 mM | 0.0295 mL | 0.1473 mL | 0.2946 mL | 0.7366 mL | |
| 80 mM | 0.0221 mL | 0.1105 mL | 0.2210 mL | 0.5525 mL | |
| 100 mM | 0.0177 mL | 0.0884 mL | 0.1768 mL | 0.4420 mL |
DBCO-CONH-S-S-NHS ester Related Classifications
HY-133413 Related Classifications
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