DCLK1-IN-2
DCLK1-IN-2 (compound I-5) is a potent DCLK1 inhibitor with an IC50 of 171.3 nM. DCLK1-IN-2 shows remarkable antiproliferative effects on SW1990 cell lines (IC50 of 0.6 μM) and in vivo antitumor potency.
For research use only. We do not sell to patients.
- CAS No.: 2978517-43-8
- Formula: C26H32N8O3S
- Molecular Weight:536.65
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CAKI-1 | IC50 |
10.1 μM
Compound: I-5
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Antiproliferative activity against human CAKI-1 cells assessed as inhibition cell proliferation incubated for 6 days by MTT assay
Antiproliferative activity against human CAKI-1 cells assessed as inhibition cell proliferation incubated for 6 days by MTT assay
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[PMID: 37862816] |
| HCT-116 | IC50 |
3 μM
Compound: I-5
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Antiproliferative activity against human HCT-116 cells assessed as inhibition cell proliferation incubated for 6 days by MTT assay
Antiproliferative activity against human HCT-116 cells assessed as inhibition cell proliferation incubated for 6 days by MTT assay
|
[PMID: 37862816] |
| SW1990 | IC50 |
0.6 μM
Compound: I-5
|
Antiproliferative activity against human SW1990 cells assessed as inhibition cell proliferation incubated for 6 days by MTT assay
Antiproliferative activity against human SW1990 cells assessed as inhibition cell proliferation incubated for 6 days by MTT assay
|
[PMID: 37862816] |
Chemical Information
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CAS No. 2978517-43-8
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Molecular Weight 536.65
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Formula C26H32N8O3S
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SMILES
CC(C)S(C1=C(NC2=NC(NC3=C(OC)C=C(N4CCN(C)CC4)C=C3)=NC5=C2N=CN5)C=CC=C1)(=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
DCLK1-IN-2 Related Classifications
HY-155303 Related Classifications
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