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Art. -Nr.: HY-D3190

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BODIPY−DOX

BODIPY-DOX is a conjugate composed of BODIPY and Doxorubicin (HY-15142A), as well as a pH-activated fluorescent probe for M1 macrophages and an apoptosis inducer. BODIPY-DOX undergoes pH-induced hydrazone bond cleavage in acidic M1 macrophage phagosomes, thereby releasing cytotoxic Doxorubicin (Dox) and inhibiting the function of pro-inflammatory M1 macrophages. BODIPY-DOX highly selectively inhibits the production of relevant pro-inflammatory cytokines by mouse and human monocyte-derived M1 macrophages, while exerting minimal effects on M2 or unactivated macrophages. Therefore, BODIPY-DOX enables simultaneous fluorescent tracing, differentiation and elimination of specific macrophage subsets, and exhibits the potential to regulate tissue regeneration in zebrafish models.

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CAS. Nr.: 2173116-67-9
Formel: C₅₄H₆₂BF₂N₇O₁₂
Molecular Weight:1049.92

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

BODIPY−DOX Chemical Structure

CAS. Nr.: 2173116-67-9

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- In Vitro
Chemical Information
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
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Bioz

Biologische Aktivität

Beschreibung

In Vitro

BODIPY-DOX (5-10 μM; overnight) induces dose-dependent cytotoxicity in LPS-polarized M1 RAW264.7 murine macrophages, but does not affect cell viability in nonactivated or IL-4-polarized M2 RAW264.7 murine macrophages^[1].
BODIPY-DOX (10 μM; 20 h) activates to release fluorescent signal and induce apoptosis specifically in LPS-polarized M1 RAW264.7 murine macrophages, with minimal effects on nonactivated RAW264.7 murine macrophages^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

LPS-polarized M1 RAW264.7 murine macrophages, IL-4-polarized M2 RAW264.7 murine macrophages, nonactivated RAW264.7 murine macrophages
Concentration:

5 μM, 10 μM
Incubation Time:

overnight
Result:

Reduced normalized cell viability of M1 macrophages to ~0.8.
Reduced normalized cell viability of M1 macrophages to ~0.65.
Did not reduce normalized cell viability in nonactivated or M2 macrophages at either concentration.

Apoptosis Analysis^[1]

Cell Line:

LPS-polarized M1 RAW264.7 murine macrophages, nonactivated RAW264.7 murine macrophages
Concentration:

10 μM
Incubation Time:

20 h
Result:

Showed strong BODIPY fluorescence staining in LPS-polarized M1 macrophages.
Increased apoptotic index (Annexin V-positive cells) of LPS-polarized M1 macrophages to 3-fold higher than that of nonactivated macrophages.
Exhibited coincident strong BODIPY fluorescence in apoptotic M1 macrophages.
Showed low BODIPY fluorescence staining and a low apoptotic index in nonactivated macrophages.

Chemical Information

CAS. Nr. 2173116-67-9
Molecular Weight 1049.92
Formel C₅₄H₆₂BF₂N₇O₁₂
SMILES

[H][C@@]1(C[C@](/C(CO)=N\NC(CCC2=[N+]3C(C(C)=C2)=C(C4=C(C=C(N4[B-]3(F)F)C)C)C5=CC=C(C=C5)NC(CN(CC)CC)=O)=O)(CC6=C(C7=C(C(O)=C61)C(C8=C(C=CC=C8C7=O)OC)=O)O)O)O[C@H]9C[C@@H]([C@@H]([C@@H](O9)C)O)N
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Reinheit & Dokumentation

Handling Instructions (2659 KB)

Verweise

[1]. Fernandez A, et al. Chemical Modulation of in Vivo Macrophage Function with Subpopulation-Specific Fluorescent Prodrug Conjugates. ACS Cent Sci. 2017;3(9):995-1005. [Content Brief]

Calculators

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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