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Cat. No.: HY-P2800

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Diaphorase, Recombinant microorganisms

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Diaphorase, Recombinant microorganisms can be obtained from anaerobic sludge organisms. Diaphorase catalyzes biotransformation of hexahydro-1,3, 5-trinitro-1,3, 5-triazine (RDX) by denitrification.

For research use only. We do not sell to patients.

CAS No.: 9001-18-7

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Diaphorase, Recombinant microorganisms Chemical Structure

CAS No.: 9001-18-7

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- EC Number
- Enzyme Activity
- Unit Definition
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Diaphorase, Recombinant microorganisms can be obtained from anaerobic sludge organisms. Diaphorase catalyzes biotransformation of hexahydro-1,3, 5-trinitro-1,3, 5-triazine (RDX) by denitrification^[1].

All Endogenous Metabolite Isoforms :

In Vitro

Product Information
Molecular Weight: 24,000
Optimal pH: 8.5. Activity is reduced by 50% at pH 7.4 and 9.4.
Inhibitor: N-ethylmaleimide inactivates diaphorase at concentrations below 5 mM

Instructions
Enzyme can be dissolved in water or 0.2 M Tris-HCl, pH 7.5. Or dissolve according to the specific experimental reference.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

EC Number

1.8.1.4

Enzyme Activity

≥30 U/mg soild

Unit Definition

One unit is defined as the amount of enzyme which oxidizes 1 μmole of NADH to NAD+ per minute at 37°C under the conditions specified in the assay procedure.

Chemical Information

CAS No. 9001-18-7
Appearance Solid
Color Light yellow to yellow
SMILES

[Diaphorase, Recombinant microorganisms]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility

In Vitro:

H₂O : 25 mg/mL (Need ultrasonic)

View the Complete Stock Solution Preparation Table

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (264 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Bhushan B, et al. Diaphorase catalyzed biotransformation of RDX via N-denitration mechanism. Biochem Biophys Res Commun. 2002 Aug 30;296(4):779-84. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

Diaphorase, Recombinant microorganisms

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Biological Activity
- Description
- In Vitro
- EC Number
- Enzyme Activity
- Unit Definition
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References

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Diaphorase, Recombinant microorganisms

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Calculators

Help & FAQs

Diaphorase, Recombinant microorganisms Related Classifications

HY-P2800 Related Classifications