Endo-β-Galactosidase
Based on 1 Customer Validation
Endo-β-Galactosidase catalyzes the hydrolysis of internal β1-4 galactose linkages in unbranched, repeating poly-N-acetyllactosamine ([GlcNAc- (1-3)Gal- (1-4)]n) structures.
For research use only. We do not sell to patients.
- CAS No.: 52720-51-1
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
others
Product Information
This product is recombinantly expressed in E. coli, with an N-terminal His-tag, a molecular weight of 36 kDa, and a buffer composition of 20 mM Tris-HCl, pH 7.5, 500 mM NaCl, 10% glycerol. It can hydrolyze the β-galactosidic bonds inside the oligosaccharide (LNnT) in the poly-N-acetyllactamine structure.
Instructions
1. Add substrate, 50 mM sodium acetate, pH 5.8, 5 mM CaCl2 to the reaction system, mix well, and add appropriate amount of endo-β-galactosidase.
2. Incubate the sample at 37°C for 2-4h or overnight.
Notes
When the system is scaled up, the incubation time or enzyme amount needs to be adjusted according to the actual situation, and can be mixed by low-speed shaking.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
3.2.1.103
≥20000 U/mL
One unit is defined as the amount of enzyme that releases 1 nmol of reducing sugar per hour from LNnT at 37°C,pH 5.8.
Chemical Information
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CAS No. 52720-51-1
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Appearance Liquid
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Color Colorless to light yellow
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SMILES
[Endo-b-Galactosidase]
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Synonyms
Keratan-sulfate endo-1,4-beta-galactosidase; Keratanase
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (266 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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