2. NF-κB MAPK/ERK Pathway Protein Tyrosine Kinase/RTK PI3K/Akt/mTOR Apoptosis Immunology/Inflammation
  3. NF-κB p38 MAPK FAK Akt Apoptosis NO Synthase COX TNF Receptor Interleukin Related
  4. Ephemeranthol A

Ephemeranthol A is a phenanthrene compound with anticancer and anti-inflammatory activities. Ephemeranthol A exerts significant anti-inflammatory effects in macrophages by inhibiting the NF-κB and MAPK signaling pathways. Ephemeranthol A induces apoptosis and inhibits metastasis of lung cancer cells by suppressing the FAK/Akt signaling and EMT processes. Ephemeranthol A can be used for the research of acute and chronic inflammatory diseases and non-small cell lung cancer.

For research use only. We do not sell to patients.

Ephemeranthol A

Ephemeranthol A Chemical Structure

CAS No. : 135545-86-7

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Ephemeranthol A Related Antibodies

Top Publications Citing Use of Products

View All NF-κB Isoform Specific Products:

View All Isoforms
NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

View All p38 MAPK Isoform Specific Products:

View All Isoforms
p38 MAPK Akt1 p38α p38β MAPK13 p38δ p38γ

View All Akt Isoform Specific Products:

View All Isoforms
Akt Akt1 Akt2 Akt3

View All NO Synthase Isoform Specific Products:

View All Isoforms
nNOS iNOS eNOS

View All COX Isoform Specific Products:

View All Isoforms
COX COX-1 COX-2 COX-3

View All TNF Receptor Isoform Specific Products:

View All Isoforms
TNFRSF1A/CD120a TNFRSF3/CD18 TNFRSF4 TNFRSF5/CD40 TNFRSF6/Fas/CD95 TNFRSF7/CD27 TNFRSF8/CD30 TNFRSF9/4-1BB/CD137 TNFRSF10B/DR5/CD262 TNFRSF12A/TWEAK TNFRSF16/NGF Receptor/CD271 TNFRSF18/GITR/CD357

View All Interleukin Related Isoform Specific Products:

View All Isoforms
IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22 IL-18

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Ephemeranthol A is a phenanthrene compound with anticancer and anti-inflammatory activities. Ephemeranthol A exerts significant anti-inflammatory effects in macrophages by inhibiting the NF-κB and MAPK signaling pathways. Ephemeranthol A induces apoptosis and inhibits metastasis of lung cancer cells by suppressing the FAK/Akt signaling and EMT processes. Ephemeranthol A can be used for the research of acute and chronic inflammatory diseases and non-small cell lung cancer[2].

IC50 & Target[1]

NF-κB

 

iNOS

 

COX-2

 

IL-1β

 

IL-6

 

In Vitro

Ephemeranthol A (6.25-50 μg/mL; 25 h) shows no cytotoxicity against Raw 264.7 cells at concentrations ≤ 25 μg/mL, but reduces cell viability at 50 μg/mL[1].
Ephemeranthol A (6.25-50 μg/mL; 7-25 h) potently inhibits LPS-induced production of NO, iNOS, COX-2, TNF-α, IL-6 and IL-1β in Raw 264.7 cells[1].
Ephemeranthol A (25 μg/mL; 1.5-2 h) blocks LPS-induced IκB degradation and inhibits the nuclear translocation of NF-κB subunits p50 and p65 in Raw 264.7 cells [1].
Ephemeranthol A (6.25-25 μg/mL; 1 h) inhibits LPS-induced phosphorylation of p38 and JNK in Raw 264.7 cells[1].
Ephemeranthol A (5-200 μM; 24-48 h) reduces the viability of human non-small cell lung cancer H460 cells in a concentration- and time-dependent manner, with an IC50 > 200 μM at 24 h and an IC50 of 150.5 μM at 48 h[2].
Ephemeranthol A (10-100 μM; 24 h) induces apoptosis in human non-small cell lung cancer H460 cells in a concentration-dependent manner[2].
Ephemeranthol A (50-100 μM; 48 h) induces apoptosis in human non-small cell lung cancer H460 cells at 48 h by decreasing Bcl-2 levels and activating caspase-9, caspase-3 and PARP[2].
Ephemeranthol A (50-100 μM; 48 h) inhibits the activation of the FAK-Akt signaling pathway in human non-small cell lung cancer H460 cells at 48 h[2].
Ephemeranthol A (5-50 μM; 48 h) inhibits anchorage-independent growth of human non-small cell lung cancer H460 cells[2].
Ephemeranthol A (10-100 μM; 24-48 h) inhibits migration of human non-small cell lung cancer H460 cells[2].
Ephemeranthol A (5-100 μM; 24-48 h) inhibits epithelial-mesenchymal transition and induces epithelial morphological changes in human non-small cell lung cancer H460 cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ephemeranthol A Related Antibodies

Western Blot Analysis[1]

Cell Line: Raw 264.7 murine macrophage cells
Concentration: 25 μg/mL
Incubation Time: 1 h pre-incubation; 6 h LPS stimulation
Result: Reduced LPS-induced iNOS protein production in Raw 264.7 cells.\n
Reduced LPS-induced COX-2 protein production in Raw 264.7 cells.

RT-PCR[1]

Cell Line: Raw 264.7 murine macrophage cells
Concentration: 25 μg/mL
Incubation Time: 1 h pre-incubation; 6 h LPS stimulation
Result: Significantly decreased LPS-induced mRNA levels of TNF-α, IL-6, and IL-1β.
Reduced TNF-α and IL-6 mRNA levels to the level of the unstimulated control.

ELISA Assay[1]

Cell Line: Raw 264.7 murine macrophage cells
Concentration: 25 μg/mL
Incubation Time: 1 h pre-incubation; 24 h LPS stimulation
Result: Significantly inhibited LPS-induced production of TNF-α, IL-6, and IL-1β protein in Raw 264.7 cells.

Western Blot Analysis[1]

Cell Line: Raw 264.7 murine macrophage cells
Concentration: 25 μg/mL
Incubation Time: 1 h pre-incubation; 30 min or 1 h LPS stimulation
Result: Blocked LPS-induced IκB degradation at 30 min post-stimulation.
Sustained the inhibitory effect until 1 h post-stimulation.\n
Inhibited LPS-induced translocation of p50 and p65 into the nucleus at 30 min post-stimulation.

Western Blot Analysis[1]

Cell Line: Raw 264.7 murine macrophage cells
Concentration: 25 μg/mL (p38, JNK phosphorylation at 10/20 min); 6.25, 12.5 and 25 μg/mL (p38 phosphorylation at 10 min)
Incubation Time: 1 h pre-incubation; 10 min or 20 min LPS stimulation (p38, JNK phosphorylation); 1 h pre-incubation, 10 min LPS stimulation (p38 phosphorylation dose-response)
Result: Reduced LPS-induced phosphorylation of p38 and JNK at 10 min and 20 min post-stimulation.
Sustained the inhibitory effect on JNK phosphorylation for 20 min.
Induced a dose-dependent reduction in LPS-induced p38 phosphorylation at 6.25, 12.5, and 25 μg/mL.

Cell Cytotoxicity Assay[2]

Cell Line: human non-small cell lung cancer H460 cells
Concentration: 5, 10, 50, 100 and 200 μM
Incubation Time: 24 h; 48 h
Result: Exhibited non-toxic to slightly toxic effects at concentrations ≤ 50 μM.
Caused significant cell viability reduction at 100 and 200 μM at both 24 and 48 h.
Reached an IC50 of > 200 μM at 24 h and 150.5 μM at 48 h.

Apoptosis Analysis[2]

Cell Line: human non-small cell lung cancer H460 cells
Concentration: 10, 50 and 100 μM
Incubation Time: 24 h
Result: Induced concentration-dependent increases in apoptotic cell number.
Resulted in a significant increase to ~17% apoptotic nuclei at 100 μM.
Kept necrotic cell numbers minimal across all concentrations.

Western Blot Analysis[2]

Cell Line: human non-small cell lung cancer H460 cells
Concentration: 50, 100 μM
Incubation Time: 48 h
Result: Increased cleaved PARP levels 1.98-fold (50 μM) and 2.33-fold (100 μM).
Increased cleaved caspase-9 levels 1.52-fold (50 μM) and 1.73-fold (100 μM).
Increased cleaved caspase-3 levels 4.78-fold (100 μM).
Reduced Bcl-2 levels to 0.63-fold (100 μM).
Showed no significant effects on Mcl-1 or Bax levels.
Reduced the p-FAK/total FAK ratio to 0.82-fold (50 μM) and 0.59-fold (100 μM).
Reduced the p-Akt/total Akt ratio to 0.52-fold (100 μM).
Kept total FAK and total Akt levels largely unchanged.

Cell Migration Assay[2]

Cell Line: human non-small cell lung cancer H460 cells
Concentration: 10, 50 and 100 μM
Incubation Time: 24 h; 48 h
Result: Showed no significant effect on wound closure at 24 h.
Reduced wound closure to 33.77% (50 μM) and 25.06% (100 μM) at 48 h, compared to untreated control wound closure of 38.40%.
Molecular Weight

272.30

Formula

C16H16O4
CAS No.
SMILES

OC1=CC2=C(C3=C(C(OC)=C(C=C3CC2)OC)O)C=C1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Ephemeranthol A Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ephemeranthol A135545-86-7NF-κBp38 MAPKFAKAktApoptosisNO SynthaseCOXTNF ReceptorInterleukin RelatedNuclear factor-κBNuclear factor-kappaBPTK2 protein tyrosine kinase 2PTK2Focal adhesion kinasePKBProtein kinase BNitric oxide synthasesNOSCyclooxygenaseTumor Necrosis Factor ReceptorTNFRILJNKnon-small cell lung cancer cellsERKiNOSmacrophagesnon-small cell lung cancerCOX-2Raw 264.7 cellsInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Ephemeranthol A
Cat. No.:
HY-N17888
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.