Ethyl 3-azidopropanoate
Based on 1 Customer Validation
Ethyl 3-azidopropanoate comprises an ethyl ester and a terminal azide group with a 2-carbon linker. The azide can react with alkyne, BCN, DBCO via Click Chemistry to yield a stable triazole linkage. The methyl ester groups can be hydrolyzed, reduced, or substituted under different conditions.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- CAS No.: 40139-55-7
- Formule: C5H9N3O2
- Masse moléculaire:143.14
-
Stockage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Activité biologique
-
Chemical Information
- Protocole
- Pureté et documentation
-
Help & FAQs
Activité biologique
Description
Chemical Information
-
CAS No. 40139-55-7
-
Appearance Liquid
-
Masse moléculaire 143.14
-
Formule C5H9N3O2
-
Color Colorless to light yellow
-
SMILES
[N-]=[N+]=NCCC(OCC)=O
-
Livraison
Room temperature in continental US; may vary elsewhere.
-
Stockage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Protocole
-
EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Pureté et documentation
-
Fiche technique (237 KB)
-
SDS (736 KB)
- English - EN (736 KB)
- Français - FR (736 KB)
- Deutsch - DE (736 KB)
- Norwegian - NO (736 KB)
- Español - ES (736 KB)
- Swedish - SV (736 KB)
- Italian - IT (736 KB)
- Korean - KR (736 KB)
- Portuguese - PT (736 KB)
-
Instruction de manipulation (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Ethyl 3-azidopropanoate Related Classifications
HY-W598198 Related Classifications
Powered by Bioz