Ferric citrate,suitable for cell culture
Based on 1 Customer Validation
Ferric citrate, suitable for cell culture is a natural preservative and food tartness enhancer. Ferric citrate, suitable for cell culture induces apoptosis and cell cycle arrest at G2/M phase and S phase in HaCaT cells. Ferric citrate, suitable for cell culture causes oxidative damage of the liver by means of the decrease of antioxidative enzyme activities. Ferric citrate, suitable for cell culture is also an acidulant, emulsifier, sequestrant and buffering agent widely used across many industries.
For research use only. We do not sell to patients.
- CAS No.: 3522-50-7
- Formula: C6H5FeO7
- Molecular Weight:244.94
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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HY-N1428C
Ferric citrate
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HY-Y0998
Potassium hexacyanoferrate(III), 99.5%
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HY-Y0416
Potassium ferrocyanide trihydrate, 98.5%
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HY-B1645
Ammonium iron(III) citrate
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HY-Y1269
Ammonium chloride, AR, 99.5%
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HY-Y1103
Iron(II) sulfate heptahydrate, 99%
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HY-Y1269C
Ammonium chloride, for cell culture
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HY-Y0406E
Calcium chloride anhydrous, for cell culture
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HY-Y1103A
Iron(II) sulfate heptahydrate, for cell culture, 99%
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HY-W019981B
Magnesium chloride hexahydrate, for molecular biology
All Endogenous Metabolite Isoforms
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Biological Activity
Ferric citrate,suitable for cell culture (0-12.5 mM; 24 h) shows antiproliferative activity in a dose dependent manner[3].
Ferric citrate,suitable for cell culture (12.5 mM; 72 h) induces apoptosis and cell cycle arrest at G2/M phase and S phase in a dosedependent manner[3].
Ferric citrate,suitable for cell culture (12.5 mM; 48 h) increases the expression of FAS, BAX, BID, AIF, EndoG, cytochrome c, PARP, GADD153, GRP78 and caspase-3, -8, -9, and decreases of BCL-2 and BCL-xL[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HaCaT cells
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Concentration:0, 2.5, 5, 7.5, 10, 12.5 mM
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Incubation Time:24 h
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Result:Inhibited the cell viability in a dose dependent manner.
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Cell Line:HaCaT cells
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Concentration:12.5 mM
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Incubation Time:0, 12, 24, 48, 72 h
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Result:Induced apoptosis and cell cycle arrest at G2/M phase and S phase in a dose-dependent manner.
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Cell Line:HaCaT cells
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Concentration:12.5 mM
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Incubation Time:12, 24, 48 h
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Result:Increased the expression of FAS, BAX, BID, AIF, EndoG, cytochrome c, PARP, GADD153, GRP78 and caspase-3, -8, -9, and decreased of BCL-2 and BCL-Xl.
Chemical Information
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CAS No. 3522-50-7
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Appearance Solid
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Molecular Weight 244.94
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Formula C6H5FeO7
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Color Yellow to brown
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SMILES
O=C(CC(C([O-])=O)(O)CC([O-])=O)[O-].[Fe+3]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
H2O : 100 mg/mL (408.26 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (277 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
[1]. Chen X, et al. Study on injury effect of food additive citric acid on liver tissue in mice. Cytotechnology. 2014 Mar;66(2):275-82. [Content Brief]
[2]. Chen X, Lv Q, Liu Y, Deng W. Effects of the food additive, citric acid, on kidney cells of mice. Biotech Histochem. 2015 Jan;90(1):38-44. [Content Brief]
[3]. Ying TH, et al. Citric acid induces cell-cycle arrest and apoptosis of human immortalized keratinocyte cell line (HaCaT) via caspase- and mitochondrial-dependent signaling pathways. Anticancer Res. 2013 Oct;33(10):4411-20. [Content Brief]
[4]. Ciriminna R, et al. Citric acid: emerging applications of key biotechnology industrial product. Chem Cent J. 2017 Mar 8;11:22. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 4.0826 mL | 20.4132 mL | 40.8263 mL | 102.0658 mL |
| 5 mM | 0.8165 mL | 4.0826 mL | 8.1653 mL | 20.4132 mL | |
| 10 mM | 0.4083 mL | 2.0413 mL | 4.0826 mL | 10.2066 mL | |
| 15 mM | 0.2722 mL | 1.3609 mL | 2.7218 mL | 6.8044 mL | |
| 20 mM | 0.2041 mL | 1.0207 mL | 2.0413 mL | 5.1033 mL | |
| 25 mM | 0.1633 mL | 0.8165 mL | 1.6331 mL | 4.0826 mL | |
| 30 mM | 0.1361 mL | 0.6804 mL | 1.3609 mL | 3.4022 mL | |
| 40 mM | 0.1021 mL | 0.5103 mL | 1.0207 mL | 2.5516 mL | |
| 50 mM | 0.0817 mL | 0.4083 mL | 0.8165 mL | 2.0413 mL | |
| 60 mM | 0.0680 mL | 0.3402 mL | 0.6804 mL | 1.7011 mL | |
| 80 mM | 0.0510 mL | 0.2552 mL | 0.5103 mL | 1.2758 mL | |
| 100 mM | 0.0408 mL | 0.2041 mL | 0.4083 mL | 1.0207 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Ferric citrate,suitable for cell culture Related Classifications
HY-N1428CA Related Classifications
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