Ferric citrate,suitable for cell culture

Name: Ferric citrate,suitable for cell culture
Brand: MedChemExpress
SKU: HY-N1428CA
Rating: 4.5 (1 reviews)

Cat. No.: HY-N1428CA: Data Sheet
COA

SDS
Handling Instructions
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Ferric citrate, suitable for cell culture is a natural preservative and food tartness enhancer. Ferric citrate, suitable for cell culture induces apoptosis and cell cycle arrest at G2/M phase and S phase in HaCaT cells. Ferric citrate, suitable for cell culture causes oxidative damage of the liver by means of the decrease of antioxidative enzyme activities. Ferric citrate, suitable for cell culture is also an acidulant, emulsifier, sequestrant and buffering agent widely used across many industries.

For research use only. We do not sell to patients.

Ferric citrate,suitable for cell culture Chemical Structure

CAS No. : 3522-50-7

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
10 g	In-stock	Estimated Time of Arrival: December 31
25 g	In-stock	Estimated Time of Arrival: December 31
50 g	In-stock	Estimated Time of Arrival: December 31
100 g	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Ferric citrate,suitable for cell culture:

Ferric citrate In-stock

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Ferric citrate,suitable for cell culture (0-12.5 mM; 24 h) shows antiproliferative activity in a dose dependent manner^[3].
Ferric citrate,suitable for cell culture (12.5 mM; 72 h) induces apoptosis and cell cycle arrest at G2/M phase and S phase in a dosedependent manner^[3].
Ferric citrate,suitable for cell culture (12.5 mM; 48 h) increases the expression of FAS, BAX, BID, AIF, EndoG, cytochrome c, PARP, GADD153, GRP78 and caspase-3, -8, -9, and decreases of BCL-2 and BCL-xL^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:	HaCaT cells
Concentration:	0, 2.5, 5, 7.5, 10, 12.5 mM
Incubation Time:	24 h
Result:	Inhibited the cell viability in a dose dependent manner.

Cell Cycle Analysis^[3]

Cell Line:	HaCaT cells
Concentration:	12.5 mM
Incubation Time:	0, 12, 24, 48, 72 h
Result:	Induced apoptosis and cell cycle arrest at G2/M phase and S phase in a dose-dependent manner.

Western Blot Analysis^[3]

Cell Line:	HaCaT cells
Concentration:	12.5 mM
Incubation Time:	12, 24, 48 h
Result:	Increased the expression of FAS, BAX, BID, AIF, EndoG, cytochrome c, PARP, GADD153, GRP78 and caspase-3, -8, -9, and decreased of BCL-2 and BCL-Xl.

Molecular Weight

244.94

Formula

C₆H₅FeO₇

CAS No.

3522-50-7

Appearance

Solid

Color

Yellow to brown

SMILES

O=C(CC(C([O-])=O)(O)CC([O-])=O)[O-].[Fe+3]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (408.26 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.0826 mL	20.4132 mL	40.8263 mL
5 mM	0.8165 mL	4.0826 mL	8.1653 mL
10 mM	0.4083 mL	2.0413 mL	4.0826 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Select Batch:

Data Sheet (277 KB) SDS (252 KB)

COA (251 KB)

Handling Instructions (2659 KB)

References

[1]. Chen X, et al. Study on injury effect of food additive citric acid on liver tissue in mice. Cytotechnology. 2014 Mar;66(2):275-82. [Content Brief]

[2]. Chen X, Lv Q, Liu Y, Deng W. Effects of the food additive, citric acid, on kidney cells of mice. Biotech Histochem. 2015 Jan;90(1):38-44. [Content Brief]

[3]. Ying TH, et al. Citric acid induces cell-cycle arrest and apoptosis of human immortalized keratinocyte cell line (HaCaT) via caspase- and mitochondrial-dependent signaling pathways. Anticancer Res. 2013 Oct;33(10):4411-20. [Content Brief]

[4]. Ciriminna R, et al. Citric acid: emerging applications of key biotechnology industrial product. Chem Cent J. 2017 Mar 8;11:22. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	4.0826 mL	20.4132 mL	40.8263 mL	102.0658 mL
	5 mM	0.8165 mL	4.0826 mL	8.1653 mL	20.4132 mL
	10 mM	0.4083 mL	2.0413 mL	4.0826 mL	10.2066 mL
	15 mM	0.2722 mL	1.3609 mL	2.7218 mL	6.8044 mL
	20 mM	0.2041 mL	1.0207 mL	2.0413 mL	5.1033 mL
	25 mM	0.1633 mL	0.8165 mL	1.6331 mL	4.0826 mL
	30 mM	0.1361 mL	0.6804 mL	1.3609 mL	3.4022 mL
	40 mM	0.1021 mL	0.5103 mL	1.0207 mL	2.5516 mL
	50 mM	0.0817 mL	0.4083 mL	0.8165 mL	2.0413 mL
	60 mM	0.0680 mL	0.3402 mL	0.6804 mL	1.7011 mL
	80 mM	0.0510 mL	0.2552 mL	0.5103 mL	1.2758 mL
	100 mM	0.0408 mL	0.2041 mL	0.4083 mL	1.0207 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.